Testing Data
Mouse CD64 Monoclonal Antibody | anti-CD64 antibody
anti-human CD64-Fab
Reactivity
Human, Dog, Primate, Monkey
Applications
Flow Cytometry, Functional Assay, Western Blot
Synonyms
CD64; Monoclonal Antibody; anti-human CD64-Fab; Monoclonal anti-human CD64 (FcgRI) Fab; anti-CD64 antibody
Host
Mouse
Reactivity
Human, Dog, Primate, Monkey
Clonality
Monoclonal
Isotype
IgG1
Clone Number
10.1
Form/Format
50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl
Concentration
1.0 mg/ml (varies by lot)
Applicable Applications for anti-CD64 antibody
Flow Cytometry (FC/FACS), Western Blot (WB)
Performance
Five x 105 cultured U-937 cells were washed and incubated 45 minutes on ice with 80 ul of anti-CD64 Fab at a concentration of 10 ug/ml. Cells were washed twice and incubated with 2 degree reagent Goat anti-Mouse/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.4 log10 fluorescent units when compared to a Mouse IgG1 Fab negative control at a similar concentration.
Conjugation
Fab
Production
Antibody was Protein A purified from (low FBS containing) tissue culture supernatant. Immunoglobulin was enzymaticly cleaved using Immobilized Ficin. Fab was separated from F(ab’)2, intact antibody and Fc fragments by Protein A and Size exclusion chromatography. Purity was >90% Fab by SDS-PAGE.
Buffer
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl. Product was sterile filtered and vialed under aseptic conditions. No preservative added.
Immunogen
Human rheumatoid synovial fluid cells/monocytes
Preparation and Storage
Store at 2 - 5 degree C. Open under aseptic conditions. Freeze/Thawing is not recommended.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Testing Data
Testing Data
Related Product Information for anti-CD64 antibody
Information: Human CD64 is a high affinity receptor for monomeric human IgG1 and IgG3 which is expressed on macrophages, monocytes, and gamma interferon induced neutrophils. CD64 plays an important role in clearance of immune complexes and in antibody dependent cytoxicity. Antibody 10.1 recognizes the CD64 molecule of 72 kd from gene Fc?RIA. Antibody 10.1 blocks binding of Fc?RI to immunoglobin opsonized cells.
References
G.J. Dougherty, et al, (1987) Eur J Immunol 17: 1453-1459. Y. Jayaram, et al, (1989) Clin Exp Immunol 75: 414-420. Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 874-875. D.L. Durden, et al, (1995) J Immunol 154: 4039-4047. L.L. Marnell, et al, (1995) J Immunol 155: 2185-2193.
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Product Notes
The CD64 (Catalog #AAA666084) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human CD64-Fab reacts with Human, Dog, Primate, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's CD64 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Western Blot (WB). Researchers should empirically determine the suitability of the CD64 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD64, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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