Immunohistochemistry (IHC)
Mouse ALY Monoclonal Antibody | anti-ALY antibody
Mouse Anti-ALY [11G5]
Reactivity
Mouse, Human, Xenopus laevis
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, ELISA, Western Blot, Immunoprecipitation, Chromatin Immunoprecipitation, Immunoprecipitation
Purity
Protein A
Synonyms
ALY; Monoclonal Antibody; Mouse Anti-ALY [11G5]; Ally of AML1 and LEF1 antibody; BEF antibody; bZIP enhancing factor antibody; REF antibody; THO complex 4antibody; THOC 4 antibody; THOC-4 antibody; THOC4 antibody; Transcriptional coactivatorantibody; anti-ALY antibody
Host
Mouse
Reactivity
Mouse, Human, Xenopus laevis
Clonality
Monoclonal
Isotype
IgG1
Clone Number
11G5
Purity/Purification
Protein A
Form/Format
Purified antibody (from supernatant) containing PBS 0.1% sodium azide;
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-ALY antibody
Flow Cytometry (FC/FACS), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP), Electron Microscopy, ChIP
Application Notes
Optimal antibody dilution should be determined by titration, however as a guideline try: IHC-P: Use a concentration of 1 ug/ml. ICC/IF: 1/50. PubMed: 17103222 WB: 1/1500. Detects a band of approximately 32 kDa (predicted molecular weight: 27 kDa).
Target Antigen
ALY
Immunogen
Recombinant human ALY / REF fusion protein
Myeloma/fusion Partners
SP2/0
Research Areas
Transcription
Immunohistochemistry (IHC)
Immunohistochemistry (IHC)
References
Ding D et al. The role of deimination in ATP5b mRNA transport in a transgenic mouse model of multiple sclerosis. EMBO Rep 13:230-6 (2012). WB, Electron Microscopy ; Mouse PMID: 22261716 Dominguez-Sanchez MS et al. Differential expression of THOC1 and ALY mRNP biogenesis/export factors in human cancers. BMC Cancer 11:77 (2011). IHC-P ; Human PMID: 21329510 Thomas M et al. The Cellular DExD/H-Box RNA-Helicases UAP56 and URH49 Exhibit a CRM1-Independent Nucleocytoplasmic Shuttling Activity. PLoS One 6:e22671 (2011). WB ; Human PMID: 21799930 Read EK & Digard P Individual influenza A virus mRNAs show differential dependence on cellular NXF1/TAP for their nuclear export. J Gen Virol 91:1290-301 (2010). WB, In Situ Hybridisation ; Human PMID: 20071484 Katahira J et al. Adaptor Aly and co-adaptor Thoc5 function in the Tap-p15-mediated nuclear export of HSP70 mRNA. EMBO J 28:556-67 (2009). WB ; Human PMID: 19165146 Taniguchi I et al. ATP-dependent recruitment of export factor Aly/REF onto intronless mRNAs by RNA helicase UAP56.Mol Cell Biol. 2008 Jan;28(2):601-8. Epub 2007 Nov 5.IP ; Xenopus laevis PMID: 17984224 van Koningsbruggen S et al. FRG1P-mediated aggregation of proteins involved in pre-mRNA processing. Chromosoma 116:53-64 (2007) PMID: 17103222 Sugiura T et al. Intracellular characterization of DDX39, a novel growth-associated RNA helicase.Exp Cell Res. 2007 Feb 15;313(4):782-90. Epub 2006 Dec 5.WB ; Human PMID: 17196963 Nojima T et al. The interaction between cap-binding complex and RNA export factor is required for intronless mRNA export. J Biol Chem 282:15645-51 (2007) PMID: 17363367 Suganuma H et al. Aly/ REF, a factor for mRNA transport, activates RH gene promoter function.FEBS J. 2005 Jun;272(11):2696-704.ChIP ; Human PMID: 15943804 The Y14 protein communicates to the cytoplasm the position of exonexon junctions. KIM VN (Embo J,Apr 2001)PMID: 11296238
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Product Notes
The ALY (Catalog #AAA375058) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-ALY [11G5] reacts with Mouse, Human, Xenopus laevis and may cross-react with other species as described in the data sheet. AAA Biotech's ALY can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP), Electron Microscopy, ChIP. Optimal antibody dilution should be determined by titration, however as a guideline try: IHC-P: Use a concentration of 1 ug/ml. ICC/IF: 1/50. PubMed: 17103222 WB: 1/1500. Detects a band of approximately 32 kDa (predicted molecular weight: 27 kDa). Researchers should empirically determine the suitability of the ALY for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ALY, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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