Testing Data
Rat anti-Mouse CD5/Lyt-1 Monoclonal Antibody
Rat Anti-Mouse CD5/Ly-1-PE
Reactivity
Mouse
Applications
Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blot
Synonyms
CD5/Lyt-1; Monoclonal Antibody; Rat Anti-Mouse CD5/Ly-1-PE; Rat Anti-Mouse CD5/Ly-1 - Phycoerythrin-Labeled; Rat Anti-Mouse CD5/Lyt-1; anti-CD5/Lyt-1 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rat IgG2a kappa
Clone Number
B19.1
Specificity
CD5/Lyt-1, Mr 67 kDa
CD5/Lyt-1 antigen is a monomeric type I transmembrane glycoprotein expressed on thymocytes, T lymphocytes, and a subset of B lymphocytes, but not on natural killer (NK) cells.^1-3 It has been identified as the major ligand of the B-cell antigen CD72.^4, 5 The frequency of CD5^+ B cells exhibits strain-dependent variation, and the phenotypic, anatomical, functional, developmental, and pathological characteristics of the CD5+ B cells suggest that they may represent a distinct lineage, known as B-1 cells (reviewed in 6). Binding of CD5 on the T cell surface can augment alloantigen- or mitogen-induced lymphocyte proliferation and induces increased cytosolic free calcium, IL-2 secretion, and IL-2R expression.^7-12 It has been proposed that CD5 negatively regulates signal transduction mediated by the T-cell and B-cell receptors.^13, 14
CD5/Lyt-1 antigen is a monomeric type I transmembrane glycoprotein expressed on thymocytes, T lymphocytes, and a subset of B lymphocytes, but not on natural killer (NK) cells.^1-3 It has been identified as the major ligand of the B-cell antigen CD72.^4, 5 The frequency of CD5^+ B cells exhibits strain-dependent variation, and the phenotypic, anatomical, functional, developmental, and pathological characteristics of the CD5+ B cells suggest that they may represent a distinct lineage, known as B-1 cells (reviewed in 6). Binding of CD5 on the T cell surface can augment alloantigen- or mitogen-induced lymphocyte proliferation and induces increased cytosolic free calcium, IL-2 secretion, and IL-2R expression.^7-12 It has been proposed that CD5 negatively regulates signal transduction mediated by the T-cell and B-cell receptors.^13, 14
Form/Format
R - phycoerythrin (R - PE) Conjugate
Applicable Applications for anti-CD5/Lyt-1 antibody
Flow Cytometry; Immunohistochemistry (Acetone Fixed/Frozen Sections); Immunoprecipitation; Western Blot
Application Notes
Research Application(s): Identification and enumeration of CD5^+ cells by flow cytometry
Identification of CD4^+ cells in acetone-fixed frozen sections
Activation of T cells in vitro
Flow Cytometry Suggested Dilution: <= 0.2 ug/10^6 cells
Identification of CD4^+ cells in acetone-fixed frozen sections
Activation of T cells in vitro
Flow Cytometry Suggested Dilution: <= 0.2 ug/10^6 cells
Characterization
To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested to conform with characteristics of a standard reference by flow cytometry. Representative data are included in this data sheet.
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg or 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The Spectral Red (SPRD) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.
The fluorescein (FITC) conjugate is supplied as 0.5 mg or 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The Spectral Red (SPRD) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.
Testing Data
Testing Data
References
1. Ledbetter, J. A. and L. A. Herzenberg. 1979. Immunol. Rev. 47:63.
2. Seaman, W. E., D. Wofsy, J. S. Greenspan, and J. A. Ledbetter. 1983. J. Immunol. 130:1713.
3. Ledbetter, J. A., R. V. Rouse, H. S. Micklem, and L. A. Herzenberg. 1980. J. Exp. Med. 152:280.
4. Van de Veld, H., I. von Hoegen, W. Luo, J. R. Parnes, and K. Thielemans. 1991. Nature 351:662.
5. Luo, W., H. Van de Velde, I. von Hoegen, J. R. Parnes, and K. Thielemans. 1992. J. Immunol. 148:1630.
6. Kantor, A. B. and L. A. Herzenberg. 1993. Ann. Rev. Immunol. 11:501.
7. Holland, N. 1982. Immunol. Rev. 68:43.
8. Stanton, T., T. L. Stevens, J. A. Ledbetter, and D. Wofsy. 1986. J. Immunol. 136:1734.
9. Verwilghen, J. R., P. Vandesande, and J. L. Ceuppens. 1996. Cell Immunol. 131:109.
10. Logdberg, L. and E. M. Shevach. 1996. Eur. J. Immunol. 15:1007.
11. June, C. H., P. R. Rabinovitch, and J. A. Ledbetter. 1987. J. Immunol. 138:2782.
12. Hollander, N., E. Pillemer, and I. L. Weissman. 1981. Proc. Natl. Acad. Sci. U. S. A. 78:1148.
13. Tarakhovsky, A., S.B. Kanner, J. Hombach, J.A. ledbetter, W. Muller, N. Killeen, and K. Rajewsky. 1995. Science 269:535.
14. Bikah, G., J. Carey, J.R. Cialella, A. Tarakhovsky, and S, Bobdada. 1996. Science 274:1906.
15. Moratz, C. and J. F. Kearney. 1996. Personal communication.
2. Seaman, W. E., D. Wofsy, J. S. Greenspan, and J. A. Ledbetter. 1983. J. Immunol. 130:1713.
3. Ledbetter, J. A., R. V. Rouse, H. S. Micklem, and L. A. Herzenberg. 1980. J. Exp. Med. 152:280.
4. Van de Veld, H., I. von Hoegen, W. Luo, J. R. Parnes, and K. Thielemans. 1991. Nature 351:662.
5. Luo, W., H. Van de Velde, I. von Hoegen, J. R. Parnes, and K. Thielemans. 1992. J. Immunol. 148:1630.
6. Kantor, A. B. and L. A. Herzenberg. 1993. Ann. Rev. Immunol. 11:501.
7. Holland, N. 1982. Immunol. Rev. 68:43.
8. Stanton, T., T. L. Stevens, J. A. Ledbetter, and D. Wofsy. 1986. J. Immunol. 136:1734.
9. Verwilghen, J. R., P. Vandesande, and J. L. Ceuppens. 1996. Cell Immunol. 131:109.
10. Logdberg, L. and E. M. Shevach. 1996. Eur. J. Immunol. 15:1007.
11. June, C. H., P. R. Rabinovitch, and J. A. Ledbetter. 1987. J. Immunol. 138:2782.
12. Hollander, N., E. Pillemer, and I. L. Weissman. 1981. Proc. Natl. Acad. Sci. U. S. A. 78:1148.
13. Tarakhovsky, A., S.B. Kanner, J. Hombach, J.A. ledbetter, W. Muller, N. Killeen, and K. Rajewsky. 1995. Science 269:535.
14. Bikah, G., J. Carey, J.R. Cialella, A. Tarakhovsky, and S, Bobdada. 1996. Science 274:1906.
15. Moratz, C. and J. F. Kearney. 1996. Personal communication.
Similar Products
Product Notes
The CD5/Lyt-1 (Catalog #AAA673172) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Rat Anti-Mouse CD5/Ly-1-PE reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD5/Lyt-1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry; Immunohistochemistry (Acetone Fixed/Frozen Sections); Immunoprecipitation; Western Blot. Research Application(s): Identification and enumeration of CD5^+ cells by flow cytometry Identification of CD4^+ cells in acetone-fixed frozen sections Activation of T cells in vitro Flow Cytometry Suggested Dilution: <= 0.2 ug/10^6 cells. Researchers should empirically determine the suitability of the CD5/Lyt-1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD5/Lyt-1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
