Mouse anti-Rat CD48 Monoclonal Antibody | anti-CD48 antibody

Anti-Rat CD48 Biotin (Clone OX-45) (mouse IgG1)

Cat. Num. AAA520170

• Gene Names: CD48; BCM1; BLAST; hCD48; mCD48; BLAST1; SLAMF2; MEM-102
• Reactivity: Rat
• Applications: Immunohistochemistry
• Synonyms: CD48; Monoclonal Antibody; Anti-Rat CD48 Biotin (Clone OX-45) (mouse IgG1); CD48 Biotin (Clone OX-45) (mouse IgG1); Biotin Anti-Rat CD48 Monoclonal Antibody; anti-CD48 antibody

• Host: Mouse
• Reactivity: Rat
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: OX-45
• Specificity: Rat CD45
• Form/Format: Biotin conjugated Ig buffered in PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml (Purified from ascites fluid via Protein G Chromatography).
• Concentration: 0.1 mg/ml (varies by lot)
• Sequence Length: 243

• Applicable Applications for anti-CD48 antibody: Immunohistochemistry (IHC)
• Application Notes: FLOW CYTOMETRY ANALYSIS:; Method:; 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Lympholyte®-Rat cell separation medium.; 2. Wash 2 times.; 3. Resuspend the cells to a concentration of 2x10⁷ cells/ml in media A. Add 50 ul of this suspension to each tube (each tube will then contain 1x10⁶ cells, representing 1 test).; 4. To each tube, add 0.2 ug* of MBS520170.; 5. Vortex the tubes to ensure thorough mixing of antibody and cells.; 6. Incubate the tubes for 30 minutes at 4°C.; 7. Wash 2 times at 4°C.; 8. Add 100 ul of secondary antibody (Streptavidin-FITC) at a 1:500 dilution.; 9. Incubate tubes at 4°C for 30 - 60 minutes (It is recommended that tubes are protected from light since most fluorochromes are light sensitive). ; 10. Wash 2 times at 4°C.; 11. Resuspend the cell pellet in 50 ul ice cold media B.; 12. Transfer to suitable tubes for flow cytometric analysis containing 15 ul of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA.; ; Media:; A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 ul of 2M sodium azide in 100 mls).; B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 ul of 2M sodium azide in 100 mls).
• Immunogen: Rat T cell blasts (stimulated purified T helper cells with allogeneic irradiated rat spleen cells)
• Donor: BALB/c spleen
• Fusion Partner: Myeloma cell line NSO/1
• Presentation: Biotin conjugated Ig buffered in PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml.
• Preparation and Storage: Store at 4 degree C. For long term storage, aliquot and freeze unused portion at -20 degree C in volumes appropriate for single usage. Avoid freeze/thaw cycles.

Testing Data

Related Product Information for anti-CD48 antibody

This anti-rat CD45 (Blast-1) monoclonal antibody recognizes a rat cell surface glycoprotein of 45 kDa that is present on a wide variety of hematopoietic cells and on endothelial cells. The antigen is identical to the mouse BCM1 antigen. This antibody inhibits allogeneic mixed lymphocyte reactions using lymph node cells as responders and spleen cells as stimulators. CD48 has recently been identified as a ligand of the NK cell inhibitory receptor CD244. This antibody is suitable for flow cytometry.

NCBI and Uniprot Product Information

• NCBI GI #: 21361571
• NCBI GeneID: 962
• NCBI Accession #: NP_001769.2
• NCBI GenBank Nucleotide #: NM_001778.3
• UniProt Accession #: P09326; Q5U055
• NCBI Official Full Name: CD48 antigen isoform 1
• NCBI Official Synonym Full Names: CD48 molecule
• NCBI Official Symbol: CD48
• NCBI Official Synonym Symbols: BCM1; BLAST; hCD48; mCD48; BLAST1; SLAMF2; MEM-102
• NCBI Protein Information: CD48 antigen; TCT.1; BCM1 surface antigen; SLAM family member 2; leukocyte antigen MEM-102; B-lymphocyte activation marker BLAST-1; CD48 antigen (B-cell membrane protein); signaling lymphocytic activation molecule 2
• UniProt Protein Name: CD48 antigen
• Protein Family: CD48 antigen
• UniProt Gene Name: CD48
• UniProt Synonym Gene Names: BCM1; BLAST1; SLAMF2
• UniProt Entry Name: CD48_HUMAN

NCBI Description

This gene encodes a member of the CD2 subfamily of immunoglobulin-like receptors which includes SLAM (signaling lymphocyte activation molecules) proteins. The encoded protein is found on the surface of lymphocytes and other immune cells, dendritic cells and endothelial cells, and participates in activation and differentiation pathways in these cells. The encoded protein does not have a transmembrane domain, however, but is held at the cell surface by a GPI anchor via a C-terminal domain which maybe cleaved to yield a soluble form of the receptor. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

Function: Ligand for CD2. Might facilitate interaction between activated lymphocytes. Probably involved in regulating T-cell activation.

Subunit structure: Interacts with CD244 in an heterophilic manner

By similarity.

Subcellular location: Cell membrane; Lipid-anchor › GPI-anchor.

Sequence similarities: Contains 2 Ig-like C2-type (immunoglobulin-like) domains.Contains 1 Ig-like V-type (immunoglobulin-like) domain.