Testing Data
Mouse anti-Human CD47 Monoclonal Antibody | anti-CD47 antibody
Monoclonal anti-human CD47/FITC
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, ELISA
Synonyms
CD47; Monoclonal Antibody; Monoclonal anti-human CD47/FITC; anti-CD47; anti-human CD47; IAP; Integrin Associated Protein; anti-CD47 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG1 kappa
Clone Number
ANC2F6
Specificity
Clone ANC2F6 binds to an epitope within the extracellular Ig-like domain of human CD47.
Form/Format
50mM Sodium Phosphate pH 7.5, 100mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2%BSA, 0.04% NaN3 (as a preservative).
Concentration
Volume in Vial: 0.2 ml (varies by lot)
Applicable Applications for anti-CD47 antibody
Flow Cytometry (FC/FACS), ELISA (EIA)
Application Notes
Working Dilution: 1:50 (or use 1.6ul of concentrated stock per 5 x 105-cell test)
Immunogen
Recombinant human CD47 (extracellular Ig domain)
Conjugation
FITC
Production
Antibody from (low FBS containing) tissue culture supernatant was Protein A purified to 95% Immunoglobulin by SDS-PAGE, and reacted with FITC. Unconjugated FITC was removed from conjugate using adesalting column. Antibody conjugate is at 0.5mg/ml with a fluorescein: antibody molar ratio of 7.1.
Performance
Five x 105 cultured CEM cells were washed and incubated 45 minutes on ice with 80ul of antiCD47/FITC at a dilution factor of 1:50 (10ug/ml). Cells were washed three times, fixed and analyzed by FACS. Cellsstained positive with a mean shift of 2.04 log10 fluorescent units when compared to a Mouse IgG1/FITC isotype control (#MBS666714). Binding was blocked when cells were preincubated 10 minutes with 20ul of 0.5mg/ml anti-CD47antibody (#MBS666928).
Preparation and Storage
Store at 2-5 degree C. Freeze/Thawing is not recommended. Protect from light
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Testing Data
Testing Data
Related Product Information for anti-CD47 antibody
Human CD47 (IAP, Integrin Associated Protein) is a broadly expressed Ig superfamily glycoprotein of about 50kD with a long extracellular Ig like domain, 5 transmembrane domains and an alternatively spliced intracellular c-terminal domain. It interacts with it ligand SIRP alpha (CD172a, Signal Regulatory Protein) expressed mainly on myeloid cells serving as a negative immune regulator, inhibiting phagocytosis. The CD47-SIRPa axis is being investigated as possible anti-tumor immune intervention point. Blockade of this interaction has shown promise in models of HNSCC (2), Glioma (3) and Breast Cancer (4)
References
1) Xiaojuan Liu, Yang-xin Fu, et al. (2017) J Hematol Oncol 10:12. doi:10.1186/s13045-016-0381-z PMID: 28077173.
2) Wu L, Sun ZJ, et al. (2018) Oncoimmunology 7 (4): e1397248. Doi: 10.1080/2162402X.2017.1397248.
3) Lif F, Jiang X, et al. (2017) Oncoimmunology 7 (2): e1391973. doi: 10.1080/2162402X.2017.1391973.
4) Norman MZ, Janji B, et al. (2018) Oncoimmunology. 7 (4):e1345415. doi: 10.1080/2162402X.2017.1345415.
2) Wu L, Sun ZJ, et al. (2018) Oncoimmunology 7 (4): e1397248. Doi: 10.1080/2162402X.2017.1397248.
3) Lif F, Jiang X, et al. (2017) Oncoimmunology 7 (2): e1391973. doi: 10.1080/2162402X.2017.1391973.
4) Norman MZ, Janji B, et al. (2018) Oncoimmunology. 7 (4):e1345415. doi: 10.1080/2162402X.2017.1345415.
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Product Notes
The CD47 (Catalog #AAA666948) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal anti-human CD47/FITC reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD47 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA). Working Dilution: 1:50 (or use 1.6ul of concentrated stock per 5 x 105-cell test). Researchers should empirically determine the suitability of the CD47 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD47, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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