Immunohistochemistry (IHC)
Mouse anti-Human CD46 Monoclonal Antibody | anti-CD46 antibody
CD46, Human, mAb M177
For unit size 0.5 mg or larger: PBS only (concentration< 0.5mg/ml)
Application Use: For immunohistology, flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For inhibition of biological activity in vitro dilutions have to be made according to the amounts of CD46 to be inactivated.
Immunohistochemistry (IHC)
Immunohistochemistry (IHC)
Four isoforms of CD46 predominate and arise by alternative splicing of a single CD46 gene. CD46 cDNA encodes a signal sequence followed by four complement control protein domains (also called short consensus repeats (SCR)). The monoclonal antibody M177 reacts with the SCR2 domain
2. Iwata, K et al; Diversity of sites for measles virus binding and for inactivation of complement C3b and C4b on membrane cofactor protein CD46. J Biol Chem 1995, 270: 15148
3. Kurita-Taniguchi, M et al; Functional modulation of human macrophages through CD46 (Measles virus receptor): production of IL-12 p40 and nitric oxide in association with recruitment of proteintyrosine phosphatase SHP-1 to CD46. J Immunol 2000, 165: 5143
4. Kurita-Taniguchi, M et al; Molecular assembly of CD46 with CD9, alpha3-beta1 integrin and protein tyrosine phosphatase SHP-1 in human macrophages through differentation by GM-CSF. Mol Immunol 2001, 38: 689
5. Lor©, K et al; Myeloid and plasmacytoid dentritic cells are susceptible to recombinant adenovirus vectors and stimulate polyfunctional memory T cell responses. J Immunol 2007, 179: 1721
6. Kato, S et al. Reduced ability of hemagglutinin of the the CAM-70 measles virus vaccine strain to use receptors CD46 and SLAM. Vaccine 2009, 27:3838
7. Matsui, H et al. Enhanced transduction efficiency of fiber-substituted adenovirus vectors by the incorporation of RGD peptides in two distict regions of the adenovirus serotype 35 fiber knob. Virus research 2010, doi10.1016/j.virusres.2010.08.021
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a type I membrane protein and is a regulatory part of the complement system. The encoded protein has cofactor activity for inactivation of complement components C3b and C4b by serum factor I, which protects the host cell from damage by complement. In addition, the encoded protein can act as a receptor for the Edmonston strain of measles virus, human herpesvirus-6, and type IV pili of pathogenic Neisseria. Finally, the protein encoded by this gene may be involved in the fusion of the spermatozoa with the oocyte during fertilization. Mutations at this locus have been associated with susceptibility to hemolytic uremic syndrome. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jun 2010]
Uniprot Description
CD46: Acts as a cofactor for complement factor I, a serine protease which protects autologous cells against complement- mediated injury by cleaving C3b and C4b deposited on host tissue. May be involved in the fusion of the spermatozoa with the oocyte during fertilization. Also acts as a costimulatory factor for T- cells which induces the differentiation of CD4+ into T-regulatory 1 cells. T-regulatory 1 cells suppress immune responses by secreting interleukin-10, and therefore are thought to prevent autoimmunity. A number of viral and bacterial pathogens seem to exploit this property and directly induce an immunosuppressive phenotype in T-cells by binding to CD46. Defects in CD46 are a cause of susceptibility to hemolytic uremic syndrome atypical type 2 (AHUS2). An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype. Patients with CD46 mutations seem to have an overall better prognosis compared to patients carrying CFH mutations. 16 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell surface; Receptor, misc.; Membrane protein, integral
Chromosomal Location of Human Ortholog: 1q32
Cellular Component: Golgi apparatus; inner acrosomal membrane; cell surface; focal adhesion; basolateral plasma membrane; integral to plasma membrane; plasma membrane
Molecular Function: complement binding; enzyme inhibitor activity; protein binding; cadherin binding; endopeptidase activity; receptor activity
Biological Process: positive regulation of memory T cell differentiation; adaptive immune response; positive regulation of regulatory T cell differentiation; viral reproduction; T cell mediated immunity; proteolysis; positive regulation of interleukin-10 production; regulation of Notch signaling pathway; negative regulation of complement activation; single fertilization; negative regulation of catalytic activity; regulation of complement activation; interleukin-10 production; innate immune response; positive regulation of T cell proliferation; complement activation, classical pathway
Research Articles on CD46
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Product Notes
The CD46 cd46 (Catalog #AAA584142) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CD46, Human, mAb M177 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD46 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FS, Immunoassay (IA), Immunoprecipitation (IP), Western Blot (WB), Immunohistochemistry (IHC). Application Notes: FC: Antibody M177 stains the extracellular domain of CD46. Application Use: For immunohistology, flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For inhibition of biological activity in vitro dilutions have to be made according to the amounts of CD46 to be inactivated. Researchers should empirically determine the suitability of the CD46 cd46 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD46, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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