Testing Data
Mouse anti-Rat CD45RC Monoclonal Antibody | anti-CD45RC antibody
Anti-Rat CD45RC Purified (Clone OX-22) (mouse IgG1)
Reactivity
Rat
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay
Synonyms
CD45RC; Monoclonal Antibody; Anti-Rat CD45RC Purified (Clone OX-22) (mouse IgG1); CD45RC Purified (Clone OX-22) (mouse IgG1); Purified Anti-Rat CD45RC Monoclonal Antibody; anti-CD45RC antibody
Host
Mouse
Reactivity
Rat
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
OX-22
Specificity
CD45RC (Rat CD45RC)
Form/Format
Purified
Concentration
Antibody Concentration: 1.0 mg/ml (varies by lot)
Applicable Applications for anti-CD45RC antibody
Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Immunogen
Phytohaemagglutinin-activated rat lymph node cells
Fusion Partner
P3-NSI-1-Ag4 (NS1/1)
Immunocyte Donor
BALB/c Spleen
Presentation
250 ug or 500 ug purified Ig buffered in PBS + 0.02% NaN3
Preparation and Storage
Stable at 4 degree C. For long term storage, aliquot and freeze unused portions at -20 degree C in volumes appropriate for single usage. Avoid repeated freeze/thaw cycles.
Testing Data
Testing Data
Related Product Information for anti-CD45RC antibody
Rat Leukocyte Common Antigen has been shown to exist in different forms on different lymphoid cell types. Rat L-CA on thymocytes gives one main band at 180 kDa, T cells show 4 bands at 180, 190, 200 and 220 kDa and B cells; one broad band at 240 kDa. T helper and T cytotoxic cell subsets show the same 4 bands with some differences in the proportions of each. MBS520166 reacts with the high molecular weight form of the leukocyte common antigen (2) which includes the 240 kDa form on B cells as well as a subfraction of the 190, 200 and 220 kDa forms, but not with the 180 kDa form of T cells or thymocytes. (5) Thus, MBS520166 reacts with B cells, cytotoxic T cells and helper cells for T cell responses but not with thymocytes or helper T cells for in vivo antibody responses. (5) Consequently, this antibody can be used to identify B cells, all CD8+ve T cells, 75% CD4+ve T cells and 50% bone marrow cells. This, antibody also divides rat CD4+ve T cells into two phenotypically distinct populations differing in their helper activities (6,7).
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Product Notes
The CD45RC (Catalog #AAA520166) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Rat CD45RC Purified (Clone OX-22) (mouse IgG1) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CD45RC can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). Researchers should empirically determine the suitability of the CD45RC for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD45RC, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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