Testing Data
Mouse anti-Rat CD5 Monoclonal Antibody | anti-CD5 antibody
Anti-Rat CD5 FITC (Clone OX-19) (mouse IgG1)
Gene Names
CD5; T1; LEU1
Reactivity
Rat
Applications
Immunohistochemistry
Synonyms
CD5; Monoclonal Antibody; Anti-Rat CD5 FITC (Clone OX-19) (mouse IgG1); CD5 FITC (Clone OX-19) (mouse IgG1); FITC Anti-Rat CD5 Monoclonal Antibody; anti-CD5 antibody
Host
Mouse
Reactivity
Rat
Clonality
Monoclonal
Isotype
Mouse IgG1
Clone Number
OX-19
Specificity
CD5 (Rat CD5)
Form/Format
FITC
Concentration
Antibody Concentration: 0.1 mg/ml (varies by lot)
Sequence Length
495
Applicable Applications for anti-CD5 antibody
Immunohistochemistry (IHC)
Immunogen
Rat thymocyte glycoproteins, depleted of LC-A, W3/13 antigen and Thy 1.
Fusion Partner
P3-NSI-I-Ag4(NS1/1)
Immunocyte Donor
BALB/c spleen
Presentation
100 ug or 500 ug FITC-labelled Ig buffered in PBS + 0.02% NaN3. BSA was added to bring total protein concentration to 4-5 mg/ml
Preparation and Storage
Stable at 4 degree C. For long term storage, aliquot and freeze unused portions at -20 degree C in volumes appropriate for single usage. Avoid repeated freeze/thaw cycles. Avoid prolonged exposure to light.
Testing Data
Testing Data
Related Product Information for anti-CD5 antibody
anti-rat CD5 monoclonal antibody recognizes a determinant expressed on all rat thymocytes and peripheral T lymphocytes. It has been determined that there are 15,000 antigenic sites for MBS520187 per cell on thymocytes and 30,000 on peripheral T cells. This antibody does not bind to B cells, macrophages, NK cells or mast cells (2). Also, no crossreactivity has been detected with non-lymphoid cells in intestine, thymus, spleen or lymph node (2). The antigen recognized by MBS520187 is a surface glycoprotein of Mr 69,000 (2), and is most likely the homologue of the mouse Ly 1 and human CD5 antigen. The antigen has also been shown to have an important functional role in T cell activation and differentiation indicating a use for this antibody in related studies. The antibody may be employed to label cells with anti-mouse Ig conjugated to fluorescein, peroxidase or 125I and has been successfully used in labelling of cryostat sections (3).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
54,578 Da
NCBI Official Full Name
T-cell surface glycoprotein CD5
NCBI Official Synonym Full Names
CD5 molecule
NCBI Official Symbol
CD5
NCBI Official Synonym Symbols
T1; LEU1
NCBI Protein Information
T-cell surface glycoprotein CD5; CD5 antigen (p56-62); lymphocyte antigen T1/Leu-1
UniProt Protein Name
T-cell surface glycoprotein CD5
Protein Family
UniProt Gene Name
CD5
UniProt Synonym Gene Names
LEU1
UniProt Entry Name
CD5_HUMAN
Uniprot Description
Function: May act as a receptor in regulating T-cell proliferation.
Subunit structure: Interacts with CD72/LYB-2. Interacts with PTPN6/SHP-1
By similarity. Ref.7
Subcellular location: Cell membrane; Single-pass type I membrane protein.
Post-translational modification: Phosphorylated on tyrosine residues by LYN; this creates binding sites for PTPN6/SHP-1
By similarity.
Sequence similarities: Contains 3 SRCR domains.
Research Articles on CD5
Similar Products
Product Notes
The CD5 cd5 (Catalog #AAA520187) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Rat CD5 FITC (Clone OX-19) (mouse IgG1) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CD5 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC). Researchers should empirically determine the suitability of the CD5 cd5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD5, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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