KAT8/MYST1 recombinant protein

Recombinant KAT8/MYST1 protein

Cat. Num. AAA389274

• Gene Names: KAT8; MOF; hMOF; MYST1; ZC2HC8
• Synonyms: KAT8/MYST1; Recombinant KAT8/MYST1 protein; MOF; Lysine Acetyltransferase 8; KAT8/MYST1 recombinant protein

• Host: Baculovirus
• Form/Format: Recombinant KAT8/MYST1 protein is supplied in 25mM HEPES-NaOH pH7.5, 300mM NaCl, 10% glycerol, 0.04% Triton X-100, and 0.5mM TCEP.

• Protein Species: Human
• Tag: DYKDDDDK-Tag
• Notes: This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data this product is shown.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant KAT8 / MYST1 protein gel 10% SDS-PAGE gel with Coomassie blue staining MW: 53.7 kDa Purity: >90%)

Western Blot (WB)

(Recombinant KAT8 / MYST1 protein activity assay 2 ug Recombinant Mononucleosomes H3.1 was incubated with 0 ug (-), 0.1 ug (+), 0.2 ug (++), and 0.4 ug (+++) KAT8 / MYST1 protein in a 20 ul reaction system containing 50 mM Tris-HCl pH 8.6, 2 mM MgCl2, 1 mM TCEP, 0.02% Triton X-100 and 20 uM Acetyl-CoA for 2 hr at RT. Reaction products were detected by Western blot using H4ac (pan-acetyl) antibody .)

Western Blot (WB)

(Recombinant KAT8 / MYST1 protein activity assay 0.5 ug Recombinant Histone H4 was incubated with 0 ug (-), 0.1 ug (+), 0.2 ug (++), and 0.4 ug (+++) KAT8 / MYST1 protein in a 20 ul reaction system containing 50 mM Tris-HCl pH 8.6, 2 mM MgCl2, 1 mM TCEP, 0.02% Triton X-100 and 20 uM Acetyl-CoA for 2 hr at RT. Reaction products were detected by Western blot using H4ac (pan-acetyl) antibody .)

Related Product Information for KAT8/MYST1 recombinant protein

Short Description: KAT8 (Lysine Acetyltransferase 8), also called as MYST1 or HMOF, is a histone acetyltransferase which may be involved in transcriptional activation. It may influence the function of ATM. As part of the MSL complex it is involved in acetylation of nucleosomal histone H4 producing specifically H4K16ac. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. That activity is less specific than the one of the MSL complex. KAT8 can also acetylate TP53/p53 at 'Lys-120'. Diseases associated with KAT8 include Atrial Septal Defect 4. Among its related pathways are Chromatin Regulation/Acetylation and Chromatin organization.

Background: KAT8 (Lysine Acetyltransferase 8), also called as MYST1 or HMOF, is a histone acetyltransferase which may be involved in transcriptional activation. It may influence the function of ATM. As part of the MSL complex it is involved in acetylation of nucleosomal histone H4 producing specifically H4K16ac. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. That activity is less specific than the one of the MSL complex. KAT8 can also acetylate TP53/p53 at 'Lys-120'. Diseases associated with KAT8 include Atrial Septal Defect 4. Among its related pathways are Chromatin Regulation/Acetylation and Chromatin organization.

Product Categories/Family for KAT8/MYST1 recombinant protein

Recombinant Proteins; Histone Acetyltransferase (HAT); Transcription

NCBI and Uniprot Product Information

• NCBI GI #: 226371635
• NCBI GeneID: 84148
• NCBI Accession #: NP_115564.2
• NCBI GenBank Nucleotide #: NM_032188
• Molecular Weight: 53,085 Da
• NCBI Official Full Name: Homo sapiens K(lysine) acetyltransferase 8 (KAT8), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: K(lysine) acetyltransferase 8
• NCBI Official Symbol: KAT8
• NCBI Official Synonym Symbols: MOF; hMOF; MYST1; ZC2HC8
• NCBI Protein Information: histone acetyltransferase KAT8; MOZ, YBF2/SAS3, SAS2 and TIP60 protein 1; MYST histone acetyltransferase 1; MYST-1; histone acetyltransferase MYST1; lysine acetyltransferase 8; ortholog of Drosophila males absent on the first (MOF); probable histone acety

NCBI Description

This gene encodes a member of the MYST histone acetylase protein family. The encoded protein has a characteristic MYST domain containing an acetyl-CoA-binding site, a chromodomain typical of proteins which bind histones, and a C2HC-type zinc finger. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]