Mouse anti-Mouse CD45.2/Ly-5.2 Monoclonal Antibody
Mouse Anti-Mouse CD45.2/Ly-5.2-APC/CY5.5
Reactivity
Mouse
Applications
Flow Cytometry, Immunoprecipitation
Synonyms
CD45.2/Ly-5.2; Monoclonal Antibody; Mouse Anti-Mouse CD45.2/Ly-5.2-APC/CY5.5; Mouse Anti-Mouse CD45.2/Ly-5.2 - Allophycocyanin/Cyanine5.5-Labeled; Mouse Anti-Mouse CD45.2/Ly-5.2; anti-CD45.2/Ly-5.2 antibody
Host
Mouse
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG2a kappa
Clone Number
104
Specificity
Mouse CD 45.2 (Leukocyte Common Antigen)
The CD45 antigen is an essential regulator of leukocyte activation and development, and is now known to be a protein tyrosine phosphatase, e.g. CD45 is able to dephosporylate the Src family of protein kinases.1, 2 This activity is now known to be required for signal transduction induced by T and B cell antigen receptor engagement, cytokine signaling and Fc receptor stimulation. 1, 2 The functional state of CD45 also has a pronounced effect on lymphocyte development. CD45 exists in multiple forms, such as the restricted variants (CD45R; see companion products 1660, 1665, 1675, 1685 and 1820) and as allelic forms CD45.1/Ly-5.1 and CD45.2/Ly-5.2. The specificity of antibodies to CD45.1 and CD45.2 has been exploited in studies where resolution of donor and recipient cells is essential, such as in gene therapy or bone marrow transplantation. 3, 4 The monoclonal antibody 104-2 recognizes CD45 (Leukocyte Common Antigen) on all leukocytes of mouse strains expressing the CD45.2 allotype (e.g. A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129).
The CD45 antigen is an essential regulator of leukocyte activation and development, and is now known to be a protein tyrosine phosphatase, e.g. CD45 is able to dephosporylate the Src family of protein kinases.1, 2 This activity is now known to be required for signal transduction induced by T and B cell antigen receptor engagement, cytokine signaling and Fc receptor stimulation. 1, 2 The functional state of CD45 also has a pronounced effect on lymphocyte development. CD45 exists in multiple forms, such as the restricted variants (CD45R; see companion products 1660, 1665, 1675, 1685 and 1820) and as allelic forms CD45.1/Ly-5.1 and CD45.2/Ly-5.2. The specificity of antibodies to CD45.1 and CD45.2 has been exploited in studies where resolution of donor and recipient cells is essential, such as in gene therapy or bone marrow transplantation. 3, 4 The monoclonal antibody 104-2 recognizes CD45 (Leukocyte Common Antigen) on all leukocytes of mouse strains expressing the CD45.2 allotype (e.g. A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, 129).
Form/Format
Allophycocyanin - Cyanine 5.5 (APC - CY5.5) Conjugate
Applicable Applications for anti-CD45.2/Ly-5.2 antibody
Flow Cytometry; Immunoprecipitation
Application Notes
Research Application(s): Markers for origin of donor and host cells
Flow Cytometry Suggested Dilution: <= 0.3 ug/10^6 cells
Flow Cytometry Suggested Dilution: <= 0.3 ug/10^6 cells
Characterization
To insure lot-to-lot consistency, each batch of product is tested for conformance with characteristics of a standard reference reagent by flow cytometry.
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) and allophycocyanin (APC) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The Spectral Red (SPRD), R-phycoerythrin-Cyanine 5.5 (R-PE-CY 5.5), R-phycoerythrin-Cyanine 7 (R-PE-CY 7), and Allophycocyanin-Cyanine 5.5 (APC-CY 5.5) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The Cyanine 5 (CY 5) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) and allophycocyanin (APC) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The Spectral Red (SPRD), R-phycoerythrin-Cyanine 5.5 (R-PE-CY 5.5), R-phycoerythrin-Cyanine 7 (R-PE-CY 7), and Allophycocyanin-Cyanine 5.5 (APC-CY 5.5) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The Cyanine 5 (CY 5) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.
References
1. Okumura, M. and M. L. Thomas. 1995. Current Opin. Immunol. 7:312-319.
2. Thomas. M. L. 1995. Seminars in Immunol. 7:279-288.
3. Mardiney, M and H. L. Malech. 1996. Blood 87:4049-4056.
4. Vallera, D. A., et al. 1994. Transplantation 57:249-256.
2. Thomas. M. L. 1995. Seminars in Immunol. 7:279-288.
3. Mardiney, M and H. L. Malech. 1996. Blood 87:4049-4056.
4. Vallera, D. A., et al. 1994. Transplantation 57:249-256.
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Product Notes
The CD45.2/Ly-5.2 (Catalog #AAA673541) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Mouse CD45.2/Ly-5.2-APC/CY5.5 reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD45.2/Ly-5.2 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry; Immunoprecipitation. Research Application(s): Markers for origin of donor and host cells Flow Cytometry Suggested Dilution: <= 0.3 ug/10^6 cells. Researchers should empirically determine the suitability of the CD45.2/Ly-5.2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD45.2/Ly-5.2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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