Rabbit SLIRP Polyclonal Antibody | anti-SLIRP antibody

SLIRP (SRA Stem-loop-interacting RNA-binding Protein Mitochondrial, Chromosome 14 Open Reading Frame 156, C14orf156, DC50, PD04872)

Cat. Num. AAA622706

• Gene Names: SLIRP; DC50; PD04872; C14orf156
• Reactivity: Human, Monkey
• Applications: Western Blot
• Purity: Affinity Purified; Purified by peptide immunoaffinity chromatography.
• Synonyms: SLIRP; Polyclonal Antibody; SLIRP (SRA Stem-loop-interacting RNA-binding Protein Mitochondrial; Chromosome 14 Open Reading Frame 156; C14orf156; DC50; PD04872); Anti -SLIRP (SRA Stem-loop-interacting RNA-binding Protein Mitochondrial; anti-SLIRP antibody

• Host: Rabbit
• Reactivity: Human, Monkey
• Clonality: Polyclonal
• Specificity: Recognizes human and monkey SLIRP protein. Does not cross-react with mouse.
• Purity/Purification: Affinity Purified; Purified by peptide immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in Tris-glycine, 150mM sodium chloride, 0.05% sodium azide

• Applicable Applications for anti-SLIRP antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 0.5-2ug/ml. Band at ~12kD corresponding to SLIRP. May see additional bands above 45kD.
• Immunogen: A synthetic peptide made to a C-terminal region of human SLIRP protein. [Entrez-Prot# AAX58600]. Homology: 100% Mk
• Cellular Localization: Nuclear
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(WB analysis for SLIRP in human liver lysate using MBS622706 at 0.6ug/ml.)

Related Product Information for anti-SLIRP antibody

SLIRP (SRA stem-loop interacting RNA binding protein) is expressed in normal and tumor tissues, but the majority of the endogenous SLIRP resides in the mitochondria. SLIRP may regulate mitochondrial functions and has the potential to shut down estrogen in breast cancer cells and testosterone in prostate cancer cells. SLIRP has also been shown to turn down genes involved in energy metabolism.

Product Categories/Family for anti-SLIRP antibody

Antibodies; Abs to Mitochondrial Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 13654278
• NCBI GeneID: 81892
• NCBI Accession #: NP_112487.1
• NCBI GenBank Nucleotide #: NM_031210.4
• UniProt Accession #: Q9GZT3
• Molecular Weight: 12,349 Da
• NCBI Official Full Name: SRA stem-loop-interacting RNA-binding protein, mitochondrial
• NCBI Official Synonym Full Names: SRA stem-loop interacting RNA binding protein
• NCBI Official Symbol: SLIRP
• NCBI Official Synonym Symbols: DC50; PD04872; C14orf156
• NCBI Protein Information: SRA stem-loop-interacting RNA-binding protein, mitochondrial
• UniProt Protein Name: SRA stem-loop-interacting RNA-binding protein, mitochondrial
• Protein Family: SRA stem-loop-interacting RNA-binding protein
• UniProt Gene Name: SLIRP
• UniProt Synonym Gene Names: C14orf156
• UniProt Entry Name: SLIRP_HUMAN

NCBI Description

Steroid receptor RNA activator (SRA, or SRA1; MIM 603819) is a complex RNA molecule containing multiple stable stem-loop structures that functions in coactivation of nuclear receptors. SLIRP interacts with stem-loop structure-7 of SRA (STR7) and modulates nuclear receptor transactivation (Hatchell et al., 2006 [PubMed 16762838]).[supplied by OMIM]

Uniprot Description

SLIRP: RNA-binding protein that acts as a nuclear receptor corepressor. Probably acts by binding the SRA RNA, and repressing the SRA-mediated nuclear receptor coactivation. Binds the STR7 loop of SRA RNA. Also able to repress glucocorticoid (GR), androgen (AR), thyroid (TR) and VDR-mediated transactivation.

Protein type: RNA-binding; Nuclear receptor co-regulator; Mitochondrial; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 14q24.3

Cellular Component: mitochondrion; perinuclear region of cytoplasm; acrosome; nucleus; ribonucleoprotein complex

Molecular Function: nucleotide binding

Biological Process: sperm motility; transcription, DNA-dependent; regulation of transcription, DNA-dependent; single fertilization; spermatid development