Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant GBP5 protein (Right) or empty vector (Left) detected with GBP5 antibody)
Mouse GBP5 Monoclonal Antibody | anti-GBP5 antibody
GBP5 antibody
Gene Names
GBP5; GBP-5
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot
Purity
GBP5 antibody was purified by affinity chromatography.
Synonyms
GBP5; Monoclonal Antibody; GBP5 antibody; Monoclonal GBP5; Anti-GBP5; Guanylate binding protein 5; Guanine nucleotide binding protein 5; GBP TA antigen; GBP 5; GBP-5; anti-GBP5 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
8D8
Purity/Purification
GBP5 antibody was purified by affinity chromatography.
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration
500 ug-1 mg/ml (varies by lot)
Sequence Length
586
Applicable Applications for anti-GBP5 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB)
Application Notes
FC: 1:100
IF: 1:100
WB: 1:2000
IF: 1:100
WB: 1:2000
Biological Significance
Like all GBPs, GBP5 has a conserved N-terminal globular GTP-binding domain containing 2 consensus sequences and a third T(L/V)RD sequence not found in other GTPases. Like GBP1 and GBP2, GBP5 has a C-terminal CaaX isoprenylation motif. EST database analysis indicated wide expression of GBP5 in human tissues. Immunofluorescence analysis demonstrated cytoplasmic expression of GBP5. Irrespective of stimulation, GBP5 was constitutively expressed in the Golgi apparatus.
Cross-Reactivity
Human
Immunogen
GBP5 antibody was raised in mouse using a full length recombinant protein of human GBP5 (NP_443174) produced in HEK293T cells, as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant GBP5 protein (Right) or empty vector (Left) detected with GBP5 antibody)
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant GBP5 protein (Right) or empty vector (Left) detected with GBP5 antibody)
Immunofluorescence (IF)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant GBP5 protein using GBP5 antibody)
Immunofluorescence (IF)
(Immunofluorescent staining of COS7 cells transiently transfected with recombinant GBP5 protein using GBP5 antibody)
Related Product Information for anti-GBP5 antibody
Mouse monoclonal GBP5 antibody
Product Categories/Family for anti-GBP5 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
55,247 Da
NCBI Official Full Name
guanylate-binding protein 5
NCBI Official Synonym Full Names
guanylate binding protein 5
NCBI Official Symbol
GBP5
NCBI Official Synonym Symbols
GBP-5
NCBI Protein Information
guanylate-binding protein 5
UniProt Protein Name
Guanylate-binding protein 5
Protein Family
UniProt Gene Name
GBP5
UniProt Synonym Gene Names
GBP-5
UniProt Entry Name
GBP5_HUMAN
Uniprot Description
GBP5: Stimulates the NLRP3 inflammasome assembly upon bacterial challenge. Belongs to the GBP family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Hydrolase; Vesicle
Chromosomal Location of Human Ortholog: 1p22.2
Cellular Component: membrane; cytoplasmic vesicle
Molecular Function: GTPase activity; identical protein binding; GTP binding
Biological Process: metabolic process
Research Articles on GBP5
Similar Products
Product Notes
The GBP5 gbp5 (Catalog #AAA831879) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's GBP5 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB). FC: 1:100 IF: 1:100 WB: 1:2000. Researchers should empirically determine the suitability of the GBP5 gbp5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GBP5, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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