Mouse anti-Human CD40 Monoclonal Antibody | anti-CD40 antibody

CD40 Antibody

Cat. Num. AAA9602759

• Gene Names: CD40; p50; Bp50; CDW40; TNFRSF5
• Reactivity: Human
• Applications: Immunofluorescence, Immunocytochemistry, ELISA
• Purity: Affinity-Chromatography
• Synonyms: CD40; Monoclonal Antibody; CD40 Antibody; AI326936; B cell associated molecule CD40; B cell surface antigen CD40; B cell-associated molecule; B-cell surface antigen CD40; Bp50; CD 40; CD40 antigen (TNF receptor superfamily member 5); CD40 antigen; CD40 molecule; TNF receptor superfamily member 5; CD40 protein; CD40 type II isoform; CD40L receptor; CDw40; GP39; HIGM1; IGM; IMD3; MGC9013; Nerve growth factor receptor related B lymphocyte activation molecule; OTTHUMP00000031699; OTTHUMP00000031700; p50; T-BAM; TBAM; TNFRSF5; TNR5_HUMAN; TRAP; Tumor necrosis factor receptor superfamily; member 5; Tumor necrosis factor receptor superfamily member 5; Tumor necrosis factor receptor superfamily member 5 precursor; isoform CRA_a; anti-CD40 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Specificity: CD40 antibody detects endogenous levels of total CD40
• Purity/Purification: Affinity-Chromatography
• Form/Format: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 277

• Applicable Applications for anti-CD40 antibody: Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
• Application Notes: ELISA: 1:10000; ICC: 1:200-1:1000
• Immunogen: Purified recombinant fragment of human CD40 expressed in E. Coli
• Subcellular Location: Secreted and Cell Membrane.
• Tissue Specificity: B-cells and in primary carcinomas.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Testing Data

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Related Product Information for anti-CD40 antibody

Description: CD40 molecule, TNF receptor superfamily member 5. It is a member of the TNF-receptor superfamily. This receptor has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
Function: Receptor for TNFSF5/CD40LG. Transduces TRAF6-and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion.
Subunit Structure: Monomer and homodimer. The variant form found in the bladder carcinoma cell line Hu549 does not form homodimers. Interacts with TRAF1, TRAF2, TRAF3, TRAF5 and TRAF6. Interacts with TRAF6 and MAP3K8; the interaction is required for ERK activation.

NCBI and Uniprot Product Information

• NCBI GI #: 4507581
• NCBI GeneID: 958
• NCBI Accession #: NP_001241.1
• NCBI GenBank Nucleotide #: NM_001250.5
• UniProt Accession #: P25942; Q53GN5; Q5JY15; Q5U007; Q7M4Q8; Q86YK5; Q9BYU0; E1P5S9
• Molecular Weight: Observed: 30 kDa; Predicted: 31 kDa
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 5 isoform 1
• NCBI Official Synonym Full Names: CD40 molecule
• NCBI Official Symbol: CD40
• NCBI Official Synonym Symbols: p50; Bp50; CDW40; TNFRSF5
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 5
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 5
• Protein Family: CD40 ligand
• UniProt Gene Name: CD40
• UniProt Synonym Gene Names: TNFRSF5

NCBI Description

This gene is a member of the TNF-receptor superfamily. The encoded protein is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Mutations affecting this gene are the cause of autosomal recessive hyper-IgM immunodeficiency type 3 (HIGM3). Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Nov 2014]

Uniprot Description

Receptor for TNFSF5/CD40LG. Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion.