Rat anti-Mouse CD40 Monoclonal Antibody

Rat Anti-Mouse CD40-PE

Cat. Num. AAA673356

• Reactivity: Mouse
• Applications: Flow Cytometry, Immunoprecipitation
• Synonyms: CD40; Monoclonal Antibody; Rat Anti-Mouse CD40-PE; Rat Anti-Mouse CD40 - Phycoerythrin-Labeled; Rat Anti-Mouse CD40; anti-CD40 antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Rat IgG2a kappa
• Clone Number: 1C10
• Specificity: Mouse CD40, Mr 45-50 kDa; CD40 is a type I cell surface protein belonging to the tumor necrosis factor superfamily of cell surface receptors. In mice it is expressed on B lineage cells, follicular dendritic cells, thymic epithelium, and interdigitating cells in the T-cell zone of secondary lymphoid organs.^1-5 CD40 first becomes detectable on a subset of small pre-B II cells in bone marrow, with the levels of CD40 expression increasing thereafter during B-cell maturation. Immature B cells (IgM^+IgD^lo B220^lo) express intermediate levels of CD40, whereas mature B cells (IgM^+IgD^hi B220^hi) express high levels.^4 CD40 has a central role in B cell growth and differentiation, and signalling through CD40 in combination with IL-4 reportedly induces immunoglobulin isotype switching and secretion of IgE.^6 The agonistic 1C10 antibody closely resembles gp39/CD40 ligand in its ability to stimulate proliferation of small, resting B lymphocytes in the absence of other cofactors.^2
• Form/Format: R - phycoerythrin (R - PE) Conjugate
• Sequence Length: 117

• Applicable Applications for anti-CD40 antibody: Flow Cytometry; Immunoprecipitation
• Application Notes: Research Application(s): In vitro induction of proliferation of mature B lymphocytes; ; Flow Cytometry Suggested Dilution: <= 0.5 ug/10^6 cells
• Characterization: To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform to characteristics of a standard reference reagent. Representative data are included in this product insert.
• Preparation and Storage: The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8 degree C. ; The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. ; The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C. ; The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! ; The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! ; The Spectral Red (SPRD) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze! ; The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C. ; Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.

References

1. Santos-Argumedo, L., J. Gordon, A.W. Heath, and M. Howard. 1994. Cell. Immunol. 156:272.
2. Heath, A.W., W.W. Wu, and M.C. Howard. 1994. Eur. J. Immunol. 24:1828.
3. Hasbold, J., C. Johnson-Leger, C.J. Atkins, E.A. Clark, and G.G. Klaus. 1994. Eur. J. Immunol. 24:1835.
4. Grandien, A., A. Bras, and C. Martinez. 1996. Scand. J. Immunol. 43:47.
5. Noelle, R.J., J.A. Ledbetter, and A. Aruffo. 1994. Immunol. Today 13:431.
6. Clark, E.A., and J.A. Ledbetter. 1994. Nature 367:425.

NCBI and Uniprot Product Information

• NCBI GI #: 114053977
• NCBI Official Full Name: CD40
• Protein Family: CD40 ligand

NCBI Description

This gene is a member of the TNF-receptor superfamily. The encoded protein is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Mutations affecting this gene are the cause of autosomal recessive hyper-IgM immunodeficiency type 3 (HIGM3). Multiple alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Nov 2014]

Uniprot Description

CD40: a member of the TNF-receptor superfamily. This receptor for CD40L mediates a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. Defects in CD40 are the cause of hyper-IgM immunodeficiency type 3 (HIGM3). HIGM3 is an autosomal recessive disorder which includes an inability of B cells to undergo isotype switching, one of the final differentiation steps in the humoral immune system, an inability to mount an antibody-specific immune response, and a lack of germinal center formation. Two alternatively spliced isoforms have been reported. Isoform I is a type I membrane protein; isoform II is secreted.

Protein type: Membrane protein, integral; Receptor, cytokine

Chromosomal Location of Human Ortholog: 20q12-q13.2

Cellular Component: extracellular space; cell surface; intracellular membrane-bound organelle; integral to plasma membrane; cytoplasm; plasma membrane; external side of plasma membrane

Molecular Function: signal transducer activity; protein binding; enzyme binding; ubiquitin protein ligase binding; receptor activity; antigen binding

Biological Process: B cell proliferation; platelet activation; positive regulation of isotype switching to IgG isotypes; regulation of immune response; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-12 production; immune response-regulating cell surface receptor signaling pathway; activation of NF-kappaB transcription factor; cellular calcium ion homeostasis; positive regulation of MAP kinase activity; positive regulation of tyrosine phosphorylation of Stat1 protein; positive regulation of B cell proliferation; protein kinase B signaling cascade; positive regulation of transcription from RNA polymerase II promoter; protein complex assembly; positive regulation of protein amino acid phosphorylation; inflammatory response; defense response to virus; regulation of immunoglobulin secretion

Disease: Immunodeficiency With Hyper-igm, Type 3