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Typical Testing Data/Standard Curve (for reference only)

Rat Trypsinogen Activation Peptide (TAP) ELISA Kit | TAP elisa kit

Rat Trypsinogen Activation Peptide (TAP) ELISA Kit

Gene Names
PRSS1; TRP1; TRY1; TRY4; TRYP1
Reactivity
Rat
Synonyms
Trypsinogen Activation Peptide (TAP); Rat Trypsinogen Activation Peptide (TAP) ELISA Kit; TAP elisa kit
Ordering
For Research Use Only!
Reactivity
Rat
Specificity
No cross-reaction with other factors.
Sequence Length
247
Samples
Rat serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant TAP concentration
Assay Type
Sandwich
Detection Range
10 ng/ml-0.156 ng/ml
Sensitivity
0.05 ng/ml.
Intra-assay Precision
<= 8%
Inter-assay Precision
<= 12%
Preparation and Storage
Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for TAP elisa kit
Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat TAP monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
26,558 Da
NCBI Official Full Name
Trypsin-1
NCBI Official Synonym Full Names
protease, serine, 1 (trypsin 1)
NCBI Official Symbol
PRSS1
NCBI Official Synonym Symbols
TRP1; TRY1; TRY4; TRYP1
NCBI Protein Information
trypsin-1; beta-trypsin; trypsinogen 1; trypsinogen A; digestive zymogen; cationic trypsinogen; nonfunctional trypsin 1
UniProt Protein Name
Trypsin-1
UniProt Gene Name
PRSS1
UniProt Synonym Gene Names
TRP1; TRY1; TRYP1
UniProt Entry Name
TRY1_HUMAN

NCBI Description

This gene encodes a trypsinogen, which is a member of the trypsin family of serine proteases. This enzyme is secreted by the pancreas and cleaved to its active form in the small intestine. It is active on peptide linkages involving the carboxyl group of lysine or arginine. Mutations in this gene are associated with hereditary pancreatitis. This gene and several other trypsinogen genes are localized to the T cell receptor beta locus on chromosome 7. [provided by RefSeq, Jul 2008]

Uniprot Description

trypsin 1: Has activity against the synthetic substrates Boc-Phe- Ser-Arg-Mec, Boc-Leu-Thr-Arg-Mec, Boc-Gln-Ala-Arg-Mec and Boc-Val- Pro-Arg-Mec. The single-chain form is more active than the two- chain form against all of these substrates. Defects in PRSS1 are a cause of pancreatitis (PCTT). A disease characterized by the presence of calculi in pancreatic ducts. It causes severe abdominal pain attacks. Belongs to the peptidase S1 family.

Protein type: Secreted, signal peptide; EC 3.4.21.4; Protease; Secreted

Chromosomal Location of Human Ortholog: 7q34

Cellular Component: extracellular region

Molecular Function: serine-type endopeptidase activity; metal ion binding

Biological Process: extracellular matrix disassembly; extracellular matrix organization and biogenesis; vitamin metabolic process; cobalamin metabolic process; digestion; proteolysis; water-soluble vitamin metabolic process

Disease: Trypsinogen Deficiency; Pancreatitis, Hereditary

Research Articles on TAP

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Product Notes

The Rat TAP prss1 (Catalog #AAA262290) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA262290 ELISA Kit recognizes Rat TAP. It is sometimes possible for the material contained within the vial of "Trypsinogen Activation Peptide (TAP), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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