Rat anti-Mouse CD4/L3T4C/CY7 Monoclonal Antibody
Rat Anti-Mouse CD4/L3T4-APC/CY7
Reactivity
Mouse
Applications
Flow Cytometry, Immunohistochemistry, Immunoprecipitation
Synonyms
CD4/L3T4C/CY7; Monoclonal Antibody; Rat Anti-Mouse CD4/L3T4-APC/CY7; Rat Anti-Mouse CD4/L3T4; anti-CD4/L3T4C/CY7 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG2b, kappa
Clone Number
GK1.5
Specificity
CD4/L3T4a, Mr 52 kDa
CD4/L3T4 is a type I transmembrane glycoprotein expressed on most thymocytes and the helper/inducer subpopulation of mature T lymphocytes of all mouse strains tested.1,2 CD4 has also been detected at low density on pluripotent hematopoietic stem cells, bone marrow myeloid precursors, and intrathymic lymphoid precursors.3-5 The CD4 antigen functions as a co-receptor, interacting with class II major histocompatability complex (MHC) molecules in the recognition of foreign antigens by T cells.1,2 In vivo and in vitro treatment with the GK1.5 mAb effectively depletes CD4+ cells.7,8 The mAb also blocks helper T cell responses to MHC class II antigens, including cytolysis, proliferation, allogeneic B cell help, and release of lymphokines.1,8-13
CD4/L3T4 is a type I transmembrane glycoprotein expressed on most thymocytes and the helper/inducer subpopulation of mature T lymphocytes of all mouse strains tested.1,2 CD4 has also been detected at low density on pluripotent hematopoietic stem cells, bone marrow myeloid precursors, and intrathymic lymphoid precursors.3-5 The CD4 antigen functions as a co-receptor, interacting with class II major histocompatability complex (MHC) molecules in the recognition of foreign antigens by T cells.1,2 In vivo and in vitro treatment with the GK1.5 mAb effectively depletes CD4+ cells.7,8 The mAb also blocks helper T cell responses to MHC class II antigens, including cytolysis, proliferation, allogeneic B cell help, and release of lymphokines.1,8-13
Form/Format
Allophycocyanin-Cyanine 7 (APC-CY 7) Conjugate
Applicable Applications for anti-CD4/L3T4C/CY7 antibody
Flow Cytometry, Immunohistochemistry (FS), Immunoprecipitation, In vivo and in vitro depletion of CD4+ cells 6,7, Fractionation of CD4+ cells14
Application Notes
Flow Cytometry: <= 0.2 ug/10^6 cells
Immunogen
Cloned mouse CTL line V41
Characterization
To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform to characteristics of a standard reference reagent. Representative data are included in this package insert.
Warning
Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Preparation and Storage
The Spectral Red (SPRD), R-phycoerythrin -Texas Red (R-PE-TXRD), R-phycoerythrin-Cyanine 7 (RPE- CY 7), R-phycoerythrin-Cyanine 5.5 (R-PE-CY 5.5 Allophycocyanin-Cyanine 5.5 (APC-CY 5.5), and Allophycocyanin- Cyanine 7 (APC-CY 7) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
References
1. Dialynas, D. P., D. B. Wilde, P. Marrack, et al. 1983. Immunol. Rev. 74:29.
2. Lakey, E. K., E. Margoliash, F. W. Fitch, and S. K. Pierce. 1986. J. Immunol. 136:3933.
3. Frederickson, G.G., and R.S. Basch. 1989. J. Exp. Med. 1969:1473.
4. Wineman, J.P., G.L. Gilmore, C. Gritzmacher, B.E. Torbett, and C.E. Muller-Sieburg. 1992. Blood 60:1717.
5. Wu, L., R. Scollay, M. Egerton, M. Pearse, G.J. Spangrude, and K. Shortman. 1991. Nature 349:71.
6. Hathcock, K.S. 1991. In Current Protocols in Immunology John Wiley & Sons, New York, p. 3.4.1.
7. Kruisbeek, A. M., et al. 1991. In Current Protocols in Immunology. John Wiley & Sons, New York, p. 4.1.1.
8. Dialynas, D. P., Z. S. Quan, K. A. Wall, et al. 1983. J. Immunol. 131:2445.
9. Swain, S. L., D. P. Dialynas, F. W. Fitch, and M. English. 1984. J. Immunol. 132:1118.
10. Havran, W. L. and F. W. Fitch. 1988. J. Immunol. 141:1808.
11. Bluestone, J.A., R.Q. Cron, T.A. Barrett, et al. 1991. Immunol. Rev. 120:5.
12. Moldwin, R. L., G. J. Nau, W. L. Havran, D. W. Lancki, and F. W. Fitch. 1987. Ann. Inst. Pasteur Immunol. 138:131.
13. Haque, S., K. Saizawa, J. Rojo, and C. A. Janeway, Jr. 1987. J. Immunol. 139:3207.
14. Mage, M. G. 1991. In Current Protocols in Immunology John Wiley and Sons, New York, p. 3.5.1.
15. B. Zheng, B., S. Han, and G. Kelsoe. 1996. J. Exp. Med 184:1083-1091.
2. Lakey, E. K., E. Margoliash, F. W. Fitch, and S. K. Pierce. 1986. J. Immunol. 136:3933.
3. Frederickson, G.G., and R.S. Basch. 1989. J. Exp. Med. 1969:1473.
4. Wineman, J.P., G.L. Gilmore, C. Gritzmacher, B.E. Torbett, and C.E. Muller-Sieburg. 1992. Blood 60:1717.
5. Wu, L., R. Scollay, M. Egerton, M. Pearse, G.J. Spangrude, and K. Shortman. 1991. Nature 349:71.
6. Hathcock, K.S. 1991. In Current Protocols in Immunology John Wiley & Sons, New York, p. 3.4.1.
7. Kruisbeek, A. M., et al. 1991. In Current Protocols in Immunology. John Wiley & Sons, New York, p. 4.1.1.
8. Dialynas, D. P., Z. S. Quan, K. A. Wall, et al. 1983. J. Immunol. 131:2445.
9. Swain, S. L., D. P. Dialynas, F. W. Fitch, and M. English. 1984. J. Immunol. 132:1118.
10. Havran, W. L. and F. W. Fitch. 1988. J. Immunol. 141:1808.
11. Bluestone, J.A., R.Q. Cron, T.A. Barrett, et al. 1991. Immunol. Rev. 120:5.
12. Moldwin, R. L., G. J. Nau, W. L. Havran, D. W. Lancki, and F. W. Fitch. 1987. Ann. Inst. Pasteur Immunol. 138:131.
13. Haque, S., K. Saizawa, J. Rojo, and C. A. Janeway, Jr. 1987. J. Immunol. 139:3207.
14. Mage, M. G. 1991. In Current Protocols in Immunology John Wiley and Sons, New York, p. 3.5.1.
15. B. Zheng, B., S. Han, and G. Kelsoe. 1996. J. Exp. Med 184:1083-1091.
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Product Notes
The CD4/L3T4C/CY7 (Catalog #AAA670336) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Rat Anti-Mouse CD4/L3T4-APC/CY7 reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD4/L3T4C/CY7 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry, Immunohistochemistry (FS), Immunoprecipitation, In vivo and in vitro depletion of CD4+ cells 6,7, Fractionation of CD4+ cells14. Flow Cytometry: <= 0.2 ug/10^6 cells. Researchers should empirically determine the suitability of the CD4/L3T4C/CY7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD4/L3T4C/CY7, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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