Rat anti-Mouse CD4 Monoclonal Antibody | anti-CD4 antibody

anti-mouse CD4-PE-Cy7

Cat. Num. AAA666217

• Gene Names: CD4; CD4mut
• Reactivity: Mouse
• Applications: Flow Cytometry, Functional Assay, ELISA, Immunohistochemistry, Immunoprecipitation
• Synonyms: CD4; Monoclonal Antibody; anti-mouse CD4-PE-Cy7; Monoclonal anti-mouse CD4/PE-Cy7; anti-CD4 antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: IgG2bk
• Clone Number: GK1.5
• Form/Format: 50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA.
• Concentration: Volume: 0.2ml (varies by lot)
• Sequence Length: 71

• Applicable Applications for anti-CD4 antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC), Immunoprecipitation (IP)
• Application Notes: 1:50 (or use 1.6ul of concentrated stock per 5 x 105-cell test)
• Florescence: PE-Cy7 is excited by 488 laser. Its Emax is 785nm.
• Performance: Reagent was tested for binding to ACK lysed murine splenocytes in FACS. Five x 105 splenocytes per tube were washed and pre incubated with 20 ul of 300 ug/ml Mouse IgG (to block non specific binding), after which they were incubated 45 minutes on ice with 80 ul of anti-Mouse CD4/PE-Cy7 at a 1:50 dilution (5 ug/ml). They were then washed three times, fixed and analyzed by FACS. A 21% sub population of the cells stained positive with a mean shift of 1.2 log10 fluorescent units when compared to background. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-mouse CD4 antibody.
• Conjugation: PE-Cy7
• Production: Protein G purified antibody from tissue culture supernatant was conjugated to Cy7-linked R-Phycoerythrin through a sulfo-ester linkage. Unconjugated antibody was removed using size exclusion chromatography. Antibody conjugate is at 250 ug/ml.
• Buffer: 50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
• Immunogen: Mouse CTL-V4 membrane prep
• Preparation and Storage: Store at 2 - 5 degree C. Do not freeze! Protect from light.; Product should retain activity for at least 12 months after shipping date when stored as recommended.

Testing Data

Related Product Information for anti-CD4 antibody

Information: Antibody GK1.5 binds to an extracellular epitope of the 55kd CD4 molecule present on a murine T cell subset (1,2). CD4 binds to MHC Class II expressed by antigen presenting cells, facilitating T Cell Receptor signaling. This clone has proven effective as a T cell depletion reagent(3).

References

1) Wilde DB, Fitch FW, et al. (1983) J Immunol 131(5):2178-2183. 2) Dialynas DP, Fitch FW, et al. (1983) J Immunol 131(5):2445-2451. 3)Mochimaru H, Ishida S, et al. (2008) Invest Ophthamol Vis Sci 49(5):2172-2177.

NCBI and Uniprot Product Information

• NCBI GI #: 3947700
• NCBI GeneID: 920
• UniProt Accession #: Q13969; P01730
• Molecular Weight: 7,844 Da
• NCBI Official Full Name: CD4, partial
• NCBI Official Synonym Full Names: CD4 molecule
• NCBI Official Symbol: CD4
• NCBI Official Synonym Symbols: CD4mut
• NCBI Protein Information: T-cell surface glycoprotein CD4; CD4 receptor; CD4 antigen (p55); T-cell surface antigen T4/Leu-3
• UniProt Protein Name: CD4 protein
• Protein Family: CD40 ligand
• UniProt Gene Name: CD4
• UniProt Entry Name: Q13969_HUMAN

NCBI Description

This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Aug 2010]

Uniprot Description

CD4: Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. Associates with LCK. Binds to HIV-1 gp120 and to P4HB/PDI and upon HIV-1 binding to the cell membrane, is part of P4HB/PDI- CD4-CXCR4-gp120 complex. Interacts with HIV-1 Envelope polyprotein gp160 and protein Vpu. Interacts with Human Herpes virus 7 capsid proteins. Interacts with PTK2/FAK1; this interaction requires the presence of HIV-1 gp120.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p13.31

Cellular Component: T cell receptor complex; endoplasmic reticulum membrane; endoplasmic reticulum lumen; early endosome; plasma membrane; integral to membrane; external side of plasma membrane; lipid raft

Molecular Function: protein binding; transmembrane receptor activity; enzyme binding; protein homodimerization activity; zinc ion binding; extracellular matrix structural constituent; coreceptor activity; receptor activity; glycoprotein binding; protein kinase binding; MHC class II protein binding

Biological Process: maintenance of cellular protein localization; viral reproduction; positive regulation of interleukin-2 biosynthetic process; T cell selection; cytokine production; positive regulation of calcium-mediated signaling; induction by virus of cell-cell fusion in host; signal transduction; defense response to Gram-negative bacterium; T cell receptor signaling pathway; enzyme linked receptor protein signaling pathway; regulation of defense response to virus by virus; positive regulation of peptidyl-tyrosine phosphorylation; cell surface receptor linked signal transduction; positive regulation of protein kinase activity; protein palmitoleylation; T cell costimulation; innate immune response; immune response; entry into host cell; transmembrane receptor protein tyrosine kinase signaling pathway; regulation of T cell activation; T cell differentiation

Disease: Okt4 Epitope Deficiency