Rat CD4 Monoclonal Antibody | anti-CD4 antibody

CD4, Mouse (T-Cell Marker)

Cat. Num. AAA4381534

• Gene Names: Cd4; L3T4; Ly-4
• Reactivity: Mouse; Does not react with Human.
• Applications: ELISA, Neutralization Assay, Flow Cytometry, Functional Assay, Immunofluorescence
• Purity: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Synonyms: CD4; Monoclonal Antibody; Mouse (T-Cell Marker); CD4 (L3T4); CD4+ Lymphocyte deficiency; included; Leu3; Ly-4; Lymphocyte antigen CD4; p55; T cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4; anti-CD4 antibody

• Host: Rat
• Reactivity: Mouse; Does not react with Human.
• Clonality: Monoclonal
• Isotype: IgG2b, kappa
• Clone Number: GK1.5
• Specificity: CD4 is a member of the Ig superfamily, primarily expressed on most thymocytes, a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a coreceptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosine kinase, lck. CD4 is expressed by the majority of thymocytes, most helper T cells, a subset of NK-T cells and weakly by dendritic cells and macrophages. CD4 plays an important role in the development of T cells and is required for mature T cells to function optimally.This antibody blocks helper T cell responses to MHC class II antigens, including cytolysis, proliferation, allogeneic B cell help, and release of lymphokines.
• Purity/Purification: Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
• Form/Format: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 457

• Applicable Applications for anti-CD4 antibody: ELISA (EIA), Neutralization Studies, Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: ELISA: For coating, order Ab without BSA & Azide; Neutralization Studies: Order Ab without BSA & Azide; FC/FACS: 0.5-1ug/million cells; IF: 0.5-1ug/ml
• Positive Control: Spleen, Thymus or Lymph Node.
• Immunogen: Mouse CTL clone V4
• Cellular Localization: Cell Surface
• Hu-chromosome Location: 6 F2; 6 59.17 cM (Mouse)
• Preparation and Storage: Antibody with azide: store at 2 to 8 degree C.; Antibody without azide: store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

References

1. Zheng B, et al. 1996. J. Exp. Med. 184:1083. (IHC). 2. Schuyler M, et al. 1994. Am. J. Respir. Crit. Care Med. 149:1286. (Deplete). 3. Gavett SH, et al. 1994. Am. J. Respir. Cell. Mol. Biol. 10:587. (Deplete). 4. Felix NJ, et al. 2007. Nat. Immunol. 8:388. (Block). 5. Godfrey DI, et al. 1994. J. Immunol. 152:4783. (Block). 6. Wu L, et al. 1991. J. Exp. Med. 174:1617. (Costim). 7. Frei K, et al. 1997. J. Exp. Med. 185:2177. (IHC)

NCBI and Uniprot Product Information

• NCBI GI #: 7304953
• NCBI GeneID: 12504
• NCBI Accession #: NP_038516.1
• NCBI GenBank Nucleotide #: NP_038516.1
• UniProt Accession #: P06332
• Molecular Weight: 55kDa
• NCBI Official Full Name: T-cell surface glycoprotein CD4
• NCBI Official Synonym Full Names: CD4 antigen
• NCBI Official Symbol: Cd4
• NCBI Official Synonym Symbols: L3T4; Ly-4
• NCBI Protein Information: T-cell surface glycoprotein CD4
• UniProt Protein Name: T-cell surface glycoprotein CD4
• Protein Family: CD40 ligand
• UniProt Gene Name: Cd4
• UniProt Entry Name: CD4_MOUSE