Rabbit anti-Human CD4 Monoclonal Antibody | anti-CD4 antibody

CD4

Cat. Num. AAA344167

• Reactivity: Human
• Synonyms: CD4; Monoclonal Antibody; anti-CD4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Form/Format: Tris Buffer, pH 7.3 - 7.7, with 1% BSA and <0.1% Sodium Azide

• Application Notes: 1:200
• Positive Control: Tonsil, Lymph Node
• Localization: Membranous
• Principles and Procedures: Visualization of the antigen present in tissue sections is accomplished in a multi-step immunohistochemical staining process, in conjunction with a horseradish peroxidase (HRP) or alkaline phosphatase (AP) linked detection system. The process involves the addition of the stated antibody (primary antibody) to a tissue slide, followed by a secondary antibody (linked to an enzyme complex) which specifically binds to the primary antibody. A chromogenic substrate is then added which reacts with the enzyme complex, resulting in a colorimetric reaction at the site of the antigen. Results are interpreted using a light microscope.
• Preparation and Storage: Store at 2-8 degree C.; To ensure stability, immediately replace vial back in the refrigerator after each use. When stored correctly, the antibody is stable until the expiry date indicated on the label.

Immunohistochemistry (IHC)

(CD4 on Tonsil)

Related Product Information for anti-CD4 antibody

Cluster of Differentiation 4 (CD4) is a membrane glycoprotein expressed in T helper cells, monocytes, macrophages, granulocytes, and dendritic cells, and is a receptor of human immunodeficiency virus (HIV). CD4 staining is used for identifying lymphoproliferative disorders. Since the majority of peripheral T-cell lymphomas arise from the T helper cell subset, CD4 expression can be found in most forms of T-cell lymphomas as well as anaplastic large T-cell lymphomas and mycosis fungoides. Since CD4 may be aberrantly expressed in neoplastic T-lymphocytes, a panel of markers may be used to identify such tumours. CD4(+) CD25(+) T-cells are reported to exert immunosuppression, which is commonly observed in various types of cancers, including non-small cell lung cancer and cancers of the breast, prostate, and ovary.