Rabbit anti-Human CD4 Monoclonal Antibody | anti-CD4 antibody

Anti-CD4 [DB81]

Cat. Num. AAA488639

• Gene Names: CD4; CD4mut
• Reactivity: Human
• Applications: ELISA, Flow Cytometry, Functional Assay, Neutralization Assay
• Purity: Purified antibody. Protein A affinity purified
• Synonyms: CD4; Monoclonal Antibody; Anti-CD4 [DB81]; Lu/T4; T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3; anti-CD4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Rabbit IgG, kappa
• Clone Number: DB81
• Specificity: This antibody is specific for human CD4, and recognizes an epitope in the vicinity of a 13-amino acid loop in domain 2 of CD4. It cross-reacts with chimpanzee CD4, but not baboon or macaque CD4.
• Purity/Purification: Purified antibody. Protein A affinity purified
• Form/Format: PBS with 0.02% Proclin 300.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-CD4 antibody: ELISA (EIA), Flow Cytometry (FC/FACS), Neutralizing (NEU)
• Application Notes: This antibody preferentially binds to a complex-enhanced epitope on the D2 domain of human CD4. Plasmon resonance analysis has shown that the binding affinity of this antibody to the CD4-gp120 complex is almost one order of magnitude higher than its binding to CD4 alone (Burastero et al, 2011), while ELISA analysis has confirmed that the epitope recognised by this antibody does not involve gp120 (Burastero et al, 2011). This antibody, through a post-binding mechanism, inhibits the cell fusion and replication of a range of HIV-1 strains (Burastero et al, 2011). It does not exert inhibitory effects on T-cell activation in vitro, a useful property for clinical use.
• Chimeric Use Statement: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
• PMID: 21818294
• Preparation and Storage: Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.

Immunofluorescence (IF)

(Immunofluorescence staining of fixed human peripheral blood leukocytes with anti-CD4 antibody DB81 (MBS488639) Immunofluorescence analysis of paraformaldehyde fixed human peripheral blood leukocytes on Shi-fix coverslips stained with the chimeric rabbit IgG version of DB81 () at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488140) followed by staining with Alexa Fluor 488 secondary antibody.)

Related Product Information for anti-CD4 antibody

Recombinant monoclonal antibody to CD4. Recombinant Platform with variable regions (i.e. specificity) from the hybridoma DB81.
This antibody was raised by immunising BALB/c mice with human CD4 complexed with HIV-1 Env (gp120).

Product Categories/Family for anti-CD4 antibody

HIV neutralisation; HIV vaccine

References

Burastero et al. Broad-Spectrum Inhibition of HIV-1 by a Monoclonal Antibody Directed against a gp120-Induced Epitope of CD4 PLoS One. 2011; 6(7): e22081.
Note on publication: Describes the original generation of this antibody, and characterisation of its binding specificity.

NCBI and Uniprot Product Information

• NCBI GI #: 10835167
• NCBI GeneID: 920
• NCBI Accession #: NP_000607.1
• NCBI GenBank Nucleotide #: NM_000616.4
• UniProt Accession #: P01730; Q4ZGK2; Q5U066; Q9UDE5; B2R737; D3DUS5
• Molecular Weight: 51,111 Da
• NCBI Official Full Name: T-cell surface glycoprotein CD4 isoform 1
• NCBI Official Synonym Full Names: CD4 molecule
• NCBI Official Symbol: CD4
• NCBI Official Synonym Symbols: CD4mut
• NCBI Protein Information: T-cell surface glycoprotein CD4; CD4 receptor; CD4 antigen (p55); T-cell surface antigen T4/Leu-3
• UniProt Protein Name: T-cell surface glycoprotein CD4
• Protein Family: CD40 ligand
• UniProt Gene Name: CD4
• UniProt Entry Name: CD4_HUMAN

NCBI Description

This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Aug 2010]

Uniprot Description

CD4: Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. Associates with LCK. Binds to HIV-1 gp120 and to P4HB/PDI and upon HIV-1 binding to the cell membrane, is part of P4HB/PDI- CD4-CXCR4-gp120 complex. Interacts with HIV-1 Envelope polyprotein gp160 and protein Vpu. Interacts with Human Herpes virus 7 capsid proteins. Interacts with PTK2/FAK1; this interaction requires the presence of HIV-1 gp120.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p13.31

Cellular Component: T cell receptor complex; endoplasmic reticulum membrane; endoplasmic reticulum lumen; early endosome; integral to membrane; plasma membrane; external side of plasma membrane; lipid raft

Molecular Function: protein binding; transmembrane receptor activity; protein homodimerization activity; enzyme binding; zinc ion binding; extracellular matrix structural constituent; coreceptor activity; receptor activity; glycoprotein binding; protein kinase binding; MHC class II protein binding

Biological Process: maintenance of cellular protein localization; viral reproduction; positive regulation of interleukin-2 biosynthetic process; cytokine production; T cell selection; positive regulation of calcium-mediated signaling; induction by virus of cell-cell fusion in host; defense response to Gram-negative bacterium; signal transduction; T cell receptor signaling pathway; enzyme linked receptor protein signaling pathway; regulation of defense response to virus by virus; positive regulation of peptidyl-tyrosine phosphorylation; cell surface receptor linked signal transduction; positive regulation of protein kinase activity; T cell costimulation; protein palmitoleylation; innate immune response; immune response; entry into host cell; transmembrane receptor protein tyrosine kinase signaling pathway; regulation of T cell activation; T cell differentiation

Disease: Okt4 Epitope Deficiency