Mouse anti-Human CD39 Monoclonal Antibody | anti-CD39 antibody

CD39 (MaxLight 650)

Cat. Num. AAA6255631

• Gene Names: ENTPD1; CD39; ATPDase; NTPDase-1
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence
• Purity: Purified by Protein G Affinity Chromatography
• Synonyms: CD39; Monoclonal Antibody; CD39 (MaxLight 650); anti-CD39 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: A1
• Specificity: Recognizes human CD39.
• Purity/Purification: Purified by Protein G Affinity Chromatography
• Form/Format: Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight650.

• Applicable Applications for anti-CD39 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Immunogen: PHA activated human lymphocytes.
• Conjugate: MaxLight650
• Preparation and Storage: Store at 4 degree C. Do Not Freeze.

Testing Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD39:FITC)

Testing Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD39 followed by Rabbit F(ab')2 anti Mouse IgG:FITC)

Product Categories/Family for anti-CD39 antibody

Antibodies; CD Markers

References

1. Aversa, G.G. et al. (1988) Detection of a late lymphocyte activation marker by A1, A new monoclonal antibody. Transpl. Proc. 20: 49-52. 2. Waugh, J.A. et al. (1989) Staining of normal and rejecting kidney using the activation panel. In: Leucocyte Typing IV. White cell differentiation antigens. Edited by Knapp, W. et al.Oxford University Press. p485. 3. Aversa, G.G. and Hall, B.M. (1989) Activation panel antigen expression on PBL activated by PHA or in MLR. In: Leucocyte Typing IV. White cell differentiation antigens. Edited by Knapp, W. et al. Oxford University Press, p.498. 4. Aversa, G.G. et al. (1989) Use of monoclonal antibodies to study in vivo and in vitro activated lymphocytes. Transpl. Proc. 21: 349-350. 5. Stein, H. et al. (1989) Activated Section report. In: Leucocyte Typing IV. White cell differentiation antigens. Edited by Knapp, W. et al. Oxford University Press, p.387. 6. Suranyi, M.G. et al. (1991) Lymphocyte adhesion molecules in T cell mediated lysis of kidney cells. Kidney International. 39: 312-319. 7. Stockl, J. et al. (2001) Monomorphic molecules function as additional recognition structures on haptenated target cells for HLA-A1 restricted, hapten-specific CTL. J. Immunol. 167: 2724-2733. 8. Scholzen, A. et al. (2009) Plasmodium falciparum-mediated induction of human CD25Foxp3 CD4 T cells is independent of direct TCR stimulation and requires IL-2, IL-10 and TGFbeta. PLoS Pathog. 5: e1000543. 9. Borsellino, G. et al. (2007) Expression of ectonucleotidase CD39 by Foxp3+ Treg cells: hydrolysis of extracellular ATP and immune suppression Blood. 110:1225-32. 10. Mittag, D. et al. (2010) The effector T cell response to ryegrass pollen is counterregulated by simultaneous induction of regulatory T cells. J Immunol. 184: 4708-16. 11. Loeuillet, C. et al. (2008) In vitro whole-genome analysis identifies a susceptibility locus for HIV-1. PLoS Biol. 6: e32. 12. Rawstron, A.C. et al. (2010) Chronic lymphocytic leukaemia (CLL) and CLL-type monoclonal B-cell lymphocytosis (MBL) show differential expression of molecules involved in lymphoid tissue homing. Cytometry B Clin Cytom. 78 Suppl 1: S42-6. 13. Alam, M.S. et al. (2009) CD73 is expressed by human regulatory T helper cells and suppresses proinflammatory cytokine production and Helicobacter felis-induced gastritis in mice. J Infect Dis. 199: 494-504. 14. Moreno-Fernandez, M.E. et al. (2011) Regulatory T cells control HIV replication in activated T cells through a cAMP-dependent mechanism. Blood. 117: 5372-80. 15. Guevara-Flores, A. et al. (2008) 5'-p-Fluorosulfonyl benzoyl adenosine inhibits an ecto-ATP- diphosphohydrolase in the tegument surface of Taenia crassiceps cysticerci. Mol Biochem Parasitol. 162: 123-33. 16. Glenn, J.R. et al. (2008) Raised levels of CD39 in leucocytosis result in marked inhibition of ADP-induced platelet aggregation via rapid ADP hydrolysis. Platelets. 19: 59-69. 17. Ha:usler, S.F.M. et al. (2014) Anti-CD39 and anti-CD73 antibodies A1 and 7G2 improve targeted therapy in ovarian cancer by blocking adenosine-dependent immune evasion. Am J Transl Res 6: 129-139.

NCBI and Uniprot Product Information

• NCBI GI #: 147905700
• NCBI GeneID: 953
• NCBI Accession #: NP_001091645.1
• NCBI GenBank Nucleotide #: NM_001098175.1
• UniProt Accession #: P49961; Q5T561; Q5T562; Q86VV3; Q9UQQ9; Q9Y3Q9; A9Z1X8; B4DWB9; B4E1X1; B7Z599; G3XAF6
• Molecular Weight: 57,965 Da
• NCBI Official Full Name: ectonucleoside triphosphate diphosphohydrolase 1 isoform 2
• NCBI Official Synonym Full Names: ectonucleoside triphosphate diphosphohydrolase 1
• NCBI Official Symbol: ENTPD1
• NCBI Official Synonym Symbols: CD39; ATPDase; NTPDase-1
• NCBI Protein Information: ectonucleoside triphosphate diphosphohydrolase 1; NTPDase 1; CD39 antigen; ecto-apyrase; ecto-ATPase 1; ecto-ATPDase 1; ecto-ATP diphosphohydrolase 1; lymphoid cell activation antigen
• UniProt Protein Name: Ectonucleoside triphosphate diphosphohydrolase 1
• UniProt Gene Name: ENTPD1
• UniProt Synonym Gene Names: CD39; NTPDase 1; Ecto-ATPDase 1
• UniProt Entry Name: ENTP1_HUMAN

NCBI Description

The protein encoded by this gene is a plasma membrane protein that hydrolyzes extracellular ATP and ADP to AMP. Inhibition of this protein's activity may confer anticancer benefits. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2015]

Uniprot Description

ENTPD1: In the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Could also be implicated in the prevention of platelet aggregation by hydrolyzing platelet-activating ADP to AMP. Hydrolyzes ATP and ADP equally well. Belongs to the GDA1/CD39 NTPase family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Nucleotide Metabolism - purine; EC 3.6.1.5; Extracellular matrix; Nucleotide Metabolism - pyrimidine; Membrane protein, multi-pass; Hydrolase

Chromosomal Location of Human Ortholog: 10q24

Cellular Component: membrane; integral to plasma membrane; plasma membrane; basal lamina

Molecular Function: protein binding; nucleoside-diphosphatase activity; nucleoside-triphosphatase activity; ATP binding

Biological Process: platelet activation; G-protein coupled receptor protein signaling pathway; purine ribonucleoside diphosphate catabolic process; cell adhesion; blood coagulation

Disease: Spastic Paraplegia 64, Autosomal Recessive