Rabbit anti-Human MCC Polyclonal Antibody | anti-MCC antibody

MCC, ID (MCC, Colorectal mutant cancer protein) (FITC)

Cat. Num. AAA6319565

• Gene Names: MCC; MCC1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MCC; Polyclonal Antibody; ID (MCC; Colorectal mutant cancer protein) (FITC); Colorectal mutant cancer protein; anti-MCC antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Sequence Length: 829

• Applicable Applications for anti-MCC antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: MCC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 472-501 amino acids from the Central region of human MCC.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(MCC Antibody (Center) western blot analysis in U937 cell line lysates (35ug/lane).This demonstrates the MCC antibody detected the MCC protein (arrow).)

Related Product Information for anti-MCC antibody

This gene is a candidate colorectal tumor suppressor gene that is thought to negatively regulate cell cycle progression. The orthologous gene in the mouse expresses a phosphoprotein associated with the plasma membrane and membrane organelles, and overexpression of the mouse protein inhibits entry into S phase. Multiple transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-MCC antibody

Antibodies; Cancer Markers

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Yoshida, T., et al. Int. J. Mol. Med. 25(4):649-656(2010). Oguri, M., et al. Am. J. Hypertens. 23(1):70-77(2010). Arnaud, C., et al. FEBS Lett. 583(14):2326-2332(2009). Fukuyama, R., et al. Oncogene 27(46):6044-6055(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 4505129
• NCBI GeneID: 4163
• NCBI Accession #: NP_002378.1; NP_001078846.1
• NCBI GenBank Nucleotide #: NP_002378.1
• UniProt Accession #: P23508
• NCBI Official Full Name: colorectal mutant cancer protein isoform 2
• NCBI Official Synonym Full Names: MCC regulator of WNT signaling pathway
• NCBI Official Symbol: MCC
• NCBI Official Synonym Symbols: MCC1
• NCBI Protein Information: colorectal mutant cancer protein
• UniProt Protein Name: Colorectal mutant cancer protein
• Protein Family: Colorectal mutant cancer protein
• UniProt Gene Name: MCC
• UniProt Synonym Gene Names: Protein MCC
• UniProt Entry Name: CRCM_HUMAN

NCBI Description

This gene is a candidate colorectal tumor suppressor gene that is thought to negatively regulate cell cycle progression. The orthologous gene in the mouse expresses a phosphoprotein associated with the plasma membrane and membrane organelles, and overexpression of the mouse protein inhibits entry into S phase. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

MCC: Candidate for the putative colorectal tumor suppressor gene located at 5q21. Suppresses cell proliferation and the Wnt/b- catenin pathway in colorectal cancer cells. Inhibits DNA binding of b-catenin/TCF/LEF transcription factors. Involved in cell migration independently of RAC1, CDC42 and p21-activated kinase (PAK) activation. Belongs to the MCC family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Tumor suppressor; Cell cycle regulation

Chromosomal Location of Human Ortholog: 5q21

Cellular Component: nucleoplasm; lamellipodium; cytoplasm; plasma membrane; nucleus

Molecular Function: protein binding; receptor activity

Biological Process: Wnt receptor signaling pathway; signal transduction; negative regulation of epithelial cell proliferation