Mouse CD34 Monoclonal Antibody | anti-CD34 antibody

Mouse Anti-Human CD34, Biotin Conjugated mAb

Cat. Num. AAA8510503

• Applications: Flow Cytometry, Functional Assay, Immunofluorescence
• Synonyms: CD34; Monoclonal Antibody; Mouse Anti-Human CD34; Biotin Conjugated mAb; anti-CD34 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG3
• Clone Number: 4A43A1
• Form/Format: Phosphate-buffered solution, pH 7.4, containing 0.09% sodium azide
• Sequence Length: 328

• Applicable Applications for anti-CD34 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Experimental Methods: 1.Take 100mul peripheral blood anticoagulated by EDTA and add to the bottom of 5ml tube; ; 2.Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature; ; 3.Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×); ; 4.Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant; ; 5.Add 2 ml PBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant; ; 6.Add appropriate amount of fluorescent-labeled Streptavidin and incubate for 20 minutes away from light at room temperature.; 7.Repeat step 5.; 8.Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 degree C then measured).; [PBS wash buffer: PBS +1% FBS +0.1% NaN3]; [Cell fixation: 2% formaldehyde solution]
• Preparation and Storage: Store at 4 degree C. DO NOT FREEZE. LIGHT SENSITIVE MATERIAL.

Related Product Information for anti-CD34 antibody

The 4A43A1 monoclonal antibody reacts with human CD34, also known as mucosialin. CD34 belongs to a protein family which also includes endoglycan and podocalyxin. Members of 4N-terminal mucin domain. CD34 was first identified as an antigen expressed on hematopoietic progenitors, and has since been extensively used as a marker to isolate cells capable of hematopoietic cell engraftment. In spite of this, the function of CD34 remains unresolved. In addition to expression on hematopoietic progenitors, CD34 is expressed on some populations of mesenchymal stem cells, tumor cell lines, and by vascular endothelia in the adult.

References

1. Schlossman, SL et al., eds. (1995) Leucocyte Typing V: White Cell Differentiation Antigens, Oxford University Press, New York. 2. Freeman SD et al., (1995) Blood 85: 2005. 3. Thomas R and Lipsky PE. (1994) J Immunol 153: 4016. 4. Nakamura Y. et al., (1994) Blood 83:1442.

NCBI and Uniprot Product Information

• NCBI GI #: 264769
• NCBI GeneID: 947
• Molecular Weight: 35,011 Da
• NCBI Official Full Name: CD34
• NCBI Official Synonym Full Names: CD34 molecule
• NCBI Official Symbol: CD34
• NCBI Protein Information: hematopoietic progenitor cell antigen CD34
• UniProt Protein Name: Hematopoietic progenitor cell antigen CD34
• Protein Family: Hematopoietic progenitor cell antigen
• UniProt Gene Name: CD34

NCBI Description

The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]

Uniprot Description

Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.