Flow Cytometry (FC/FACS)
(Flow cytometric analysis of TF1 cells using Anti-CD326 Antibody, followed by anti-mouse IgG PE.)
Mouse anti-Human CD326 Monoclonal Antibody | anti-EPCAM antibody
Anti-CD326 Antibody
2.- Add aproppiate amount of antibody to the bottom of flow tube mixing with the blood; incubate for 30 minutes at room temperature.
3.- Add 2mL RBC lysis buffer; incubate for 10 minutes after mixing; dissolve red blood cells.
4.- Sample tube is set to 1000 rpm centrifugation for 5 minutes; discard the supernatant.
5.- Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant.
6.- Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature.
7.- Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes; discard the supernatant.
8. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4°C then measured).
Store at 4°C for one year. Do not freeze.
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of TF1 cells using Anti-CD326 Antibody, followed by anti-mouse IgG PE.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of TF1 cells using Anti-CD326 Antibody, followed by anti-mouse IgG PE.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008]
Uniprot Description
EPCAM: a single-pass type I membrane protein of the EPCAM family. May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinomas including pancreatic carcinoma. May be a marker of cancer stem cells.
Protein type: Membrane protein, integral; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 2p21
Cellular Component: cell surface; tight junction; basolateral plasma membrane; apical plasma membrane; integral to membrane; plasma membrane; lateral plasma membrane
Molecular Function: protein binding; protein complex binding
Biological Process: ureteric bud development; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; stem cell differentiation
Disease: Diarrhea 5, With Tufting Enteropathy, Congenital; Colorectal Cancer, Hereditary Nonpolyposis, Type 8
Research Articles on EPCAM
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Product Notes
The EPCAM epcam (Catalog #AAA8204495) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD326 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD326 can be used in a range of immunoassay formats including, but not limited to, Immunofluoresence (IF), Flow Cytometry (FC/FACS). Researchers should empirically determine the suitability of the EPCAM epcam for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD326, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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