Testing Data
(Staining of bovine peripheral blood lymphocytes with Mouse anti Bovine CD32 (MBS2130998GA) followed by Goat anti Mouse IgG:FITC (MBS235142))
Mouse CD32 Monoclonal Antibody | anti-CD32 antibody
MOUSE ANTI BOVINE CD32:RPE
Gene Names
FCGR2B; CD32; FCGR2; fcRII
Applications
Flow Cytometry, Functional Assay
Synonyms
CD32; Monoclonal Antibody; MOUSE ANTI BOVINE CD32:RPE; anti-CD32 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
CCG36
Form/Format
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
Sequence Length
296
Applicable Applications for anti-CD32 antibody
Flow cytometry (FC/FACS)
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry: Maximum Dilution: Neat
Flow Cytometry: Maximum Dilution: Neat
Reconstitution
Reconstitute with 1.0 ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution.
Perservative Stabilisers
Perservative Stabilisers
Immunogen
Bovine Fc gamma RII-transfected COS7 cells
Fusion Partners
Fusion Partners
Target Species
Bovine
Preparation and Storage
Prior to reconstitution store at 4 degree C. After reconstitution store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light.
Shelf Life: 18 months from date of reconstitution
Shelf Life: 18 months from date of reconstitution
Testing Data
(Staining of bovine peripheral blood lymphocytes with Mouse anti Bovine CD32 (MBS2130998GA) followed by Goat anti Mouse IgG:FITC (MBS235142))
Testing Data
(Staining of bovine peripheral blood lymphocytes with Mouse anti Bovine CD32 (MBS2130998GA) followed by Goat anti Mouse IgG:FITC (MBS235142))
Testing Data
(Staining of bovine peripheral blood lymphocytes with Mouse anti Bovine CD32:FITC (MBS2130998F))
Testing Data
(Staining of bovine peripheral blood lymphocytes with Mouse anti Bovine CD32:FITC (MBS2130998F))
Testing Data
(Staining of bovine peripheral blood lymphocytes with Mouse anti Bovine CD32:RPE)
Testing Data
(Staining of bovine peripheral blood lymphocytes with Mouse anti Bovine CD32:RPE)
Related Product Information for anti-CD32 antibody
Mouse anti Bovine CD32 antibody, clone CCG36 recognizes the bovine homologue of human CD32, one of a group of Fc receptors belonging to the immunoglobulin superfamily and involved in phagocytosis of opsonized microbes. Bovine CD32 is a single pass type 1 membrane protein of approximately 32kDa, expressed on the cell surface of most cells including B-lymphocytes, monocytes, neutrophils and afferent veiled lymph dendritic cells Chattha, K. et al. 2010. It has been shown that expression of bovine CD32 is higher on macrophages than on neutrophils. CD32 can function in an inhibitory capacity to antibody production and is the low affinity Fc receptor for IgG (FcRII), binding to the Fc region of immunoglobulin gamma Chattha et al. 2009. Mouse anti Bovine CD32, clone CCG36 is one of a number of anti bovine CD32 reagents available from MyBioSource, clone CCG36 is of interest in that it also recognizes ovine CD32 while clone CCG39 recognizes only bovine CD32.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
33,020 Da
NCBI Official Full Name
low affinity immunoglobulin gamma Fc region receptor II-b
NCBI Official Symbol
FCGR2B
NCBI Official Synonym Symbols
CD32; FCGR2; fcRII
NCBI Protein Information
low affinity immunoglobulin gamma Fc region receptor II-b; fc-gamma RII; igG Fc receptor II
UniProt Protein Name
Low affinity immunoglobulin gamma Fc region receptor II
Protein Family
UniProt Gene Name
FCGR2
UniProt Synonym Gene Names
IgG Fc receptor II; FcRII
UniProt Entry Name
FCGR2_BOVIN
Uniprot Description
Binds to the Fc region of immunoglobulins gamma. Low affinity receptor.
Similar Products
Product Notes
The CD32 fcgr2 (Catalog #AAA219877) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD32 can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS). Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul Flow Cytometry: Maximum Dilution: Neat. Researchers should empirically determine the suitability of the CD32 fcgr2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD32, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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