CD113/NECTIN3 recombinant protein

CD113/NECTIN3 Recombinant Protein

Cat. Num. AAA3003829

• Gene Names: PVRL3; PPR3; PRR3; CD113; PVRR3; CDW113; NECTIN-3
• Purity: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is >85% (by SDS-PAGE).
• Synonyms: CD113/NECTIN3; CD113/NECTIN3 Recombinant Protein; CD113/NECTIN3 recombinant protein

• Host: E Coli
• Purity/Purification: Transferred into competent cells and the supernatant was purified by NI column affinity chromatography and the purity is >85% (by SDS-PAGE).
• Form/Format: PBS, 4M Urea, PH7.4
• Concentration: >=0.5mg/ml (varies by lot)
• Sequence: GPIIVEPHVTAVWGKNVSLKCLIEVNETITQISWEKIHGKSSQTVAVHHPQYGFSVQGEYQGRVLFKNYSLNDATITLHNIGFSDSGKYICKAVTFPLGNAQSSTTVTVLVEPTVSLIKGPDSLIDGGNETVAAICIAATGKPVAHIDWEGDLGEMESTTTSFPNETATIISQYKLFPTRFARGRRITCVVKHPALEKDIRYSFILDIQYAPEVSVTGYDGNWFVGRKGVNLKCNADANPPPFKSVWSRLDGQWPDGLLASDNTLHFVHPLTFNYSGVYICKVTNSLGQRSDQKVIYISDPPTTTTLQPTIQWHPSTADIEDLATEPKKLPFPLSTLATIKDDTIAT

• Tag: His-tag in C-terminal
• Expression Vector: pet-22b(+)
• BP: 1053
• Restriction Site: NdeI-XhoI
• Preparation and Storage: Store at 4 degee C for short term. Aliquot and store at -20 degee C long term. Avoid freeze-thaw cycles.

Testing Data

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Related Product Information for CD113/NECTIN3 recombinant protein

Background: Nectin is a Ca2+-independent homophilic cell adhesion molecule that belongs to the immunoglobulin superfamily. Human Nectin is identical to the poliovirus receptor-related protein (PRR) and is identified to be the alphaherpesvirus entry mediator. Nectin constitutes a family consisting of at least Nectin 1, 2 and 3. Nectin 2 and 3 are ubiquitously expressed, whereas Nectin 1 is abundantly expressed in brain. Nectin 3, also designated as PRR3, has three splicing variants: Nectin 3alpha, 3beta and 3gamma. Nectin 3 is a transmembrane protein whose extracellular region contains three Ig-like domains. Nectin 3alpha and 3beta, but not Nectin 3gamma, have a C-terminal conserved motif (E/A-X-Y-V). This motif interacts with the PDZ domain of the F-Actin-binding protein, afadin, through which it is linked to the Actin cytoskeleton. Nectin 3alpha co-localizes with Nectin 2 at cadherin-based adheren junctions and interacts with afadin.

NCBI and Uniprot Product Information

• NCBI GI #: 343168783
• NCBI GeneID: 25945
• NCBI Accession #: NP_001230215.1
• NCBI GenBank Nucleotide #: NM_001243286.1
• UniProt Accession #: Q9NQS3; Q6NVZ3; Q8WVU4; Q9BVA9; Q9Y412
• Molecular Weight: 61,002 Da
• NCBI Official Full Name: poliovirus receptor-related protein 3 isoform 2
• NCBI Official Synonym Full Names: poliovirus receptor-related 3
• NCBI Official Symbol: PVRL3
• NCBI Official Synonym Symbols: PPR3; PRR3; CD113; PVRR3; CDW113; NECTIN-3
• NCBI Protein Information: poliovirus receptor-related protein 3; nectin 3
• UniProt Protein Name: Poliovirus receptor-related protein 3
• UniProt Gene Name: PVRL3
• UniProt Synonym Gene Names: PRR3
• UniProt Entry Name: PVRL3_HUMAN

NCBI Description

This gene encodes a member of the nectin family of proteins, which function as adhesion molecules at adherens junctions. This family member interacts with other nectin-like proteins and with afadin, a filamentous actin-binding protein involved in the regulation of directional motility, cell proliferation and survival. This gene plays a role in ocular development involving the ciliary body. Mutations in this gene are believed to result in congenital ocular defects. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2011]

Uniprot Description

PVRL3: Plays a role in cell-cell adhesion through heterophilic trans-interactions with nectin-like proteins or nectins, such as trans-interaction with PVRL2/nectin-2 at Sertoli-spermatid junctions. Trans-interaction with PVR induces activation of CDC42 and RAC small G proteins through common signaling molecules such as SRC and RAP1. Also involved in the formation of cell-cell junctions, including adherens junctions and synapses. Induces endocytosis-mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation. Plays a role in the morphology of the ciliary body. Belongs to the nectin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3q13

Cellular Component: apical junction complex; postsynaptic membrane; cell-cell adherens junction; plasma membrane; integral to membrane

Molecular Function: protein binding; protein homodimerization activity; cell adhesion molecule binding

Biological Process: intercellular junction assembly and maintenance; cell-cell adhesion; fertilization; lens morphogenesis in camera-type eye; homophilic cell adhesion; retina morphogenesis in camera-type eye