Flow Cytometry (FC/FACS)
(Staining of normal human peripheral blood cells with Mouse IgG2a K Isotype Control (left) or CD172a antibody (right). Cells in the lymphocyte (green histogram) or granulocyte (blue histogram) gate were used for analysis.)
Mouse CD172a Monoclonal Antibody | anti-CD172a antibody
CD172a antibody (allophycocyanin)
Applications
Flow Cytometry, Functional Assay
Synonyms
CD172a; Monoclonal Antibody; CD172a antibody (allophycocyanin); Monoclonal CD172a; Anti-CD172a; CD172a (APC); Tyrosine-protein phosphatase non-receptor type substrate 1; SHPS-1; CD-172a; CD 172a; SIRPa; anti-CD172a antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
15-414
Specificity
Mouse
Form/Format
Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration
200 ug/ml (varies by lot)
Applicable Applications for anti-CD172a antibody
Flow Cytometry (FC/FACS)
Application Notes
FC: <= 0.06 ug/test
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Biological Significance
This 15-414 monoclonal antibody reacts with human CD172a, also known as signal regulatory protein alpha. This cell surface glycoprotein consists of three Ig-like extracellular domains and two cytoplasmic immunoreceptor tyrosine-based inhibitory motifs. The ITIM domains have been demonstrated to recruit and bind the Src homology 2 domain-containing phosphatases SHP-1 and SHP-2. CD172a is expressed on monocytes, granulocytes, dendritic cells, and CD34CD38- hematopoietic progenitor cells but not on T and B lymphocytes. Moreover, neurons have also been shown to express CD172. The integrin-associated protein CD47 is the extracellular ligand for CD172a. Studies show that CD172a is involved in dendritic cell-mediated T cell activation, neutrophil migration, and phagocytosis.
Tag/Conjugate
Allophycocyanin
Preparation and Storage
Store at 2-8 degree C. Do not freeze. Protect from light.
Flow Cytometry (FC/FACS)
(Staining of normal human peripheral blood cells with Mouse IgG2a K Isotype Control (left) or CD172a antibody (right). Cells in the lymphocyte (green histogram) or granulocyte (blue histogram) gate were used for analysis.)
Flow Cytometry (FC/FACS)
(Staining of normal human peripheral blood cells with Mouse IgG2a K Isotype Control (left) or CD172a antibody (right). Cells in the lymphocyte (green histogram) or granulocyte (blue histogram) gate were used for analysis.)
Related Product Information for anti-CD172a antibody
Mouse monoclonal CD172a antibody (allophycocyanin)
Product Categories/Family for anti-CD172a antibody
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Product Notes
The CD172a (Catalog #AAA832885) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD172a can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC: <= 0.06 ug/test A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Researchers should empirically determine the suitability of the CD172a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD172a, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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