Mouse CD31/PECAM-1 Monoclonal Antibody | anti-CD31 antibody

CD31/PECAM-1 (Endothelial Cell Marker)

Cat. Num. AAA4380148

• Gene Names: PECAM1; CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM
• Reactivity: Human. Others not known.
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence
• Synonyms: CD31/PECAM-1; Monoclonal Antibody; CD31/PECAM-1 (Endothelial Cell Marker); EndoCAM; PECA1; Platelet Endothelial Cell Adhesion Molecule 1; GPIIA'; anti-CD31 antibody

• Host: Mouse
• Reactivity: Human. Others not known.
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: C31.12
• Specificity: CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 MAb reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 738

• Applicable Applications for anti-CD31 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: FC/FACS: 0.5-1ug/million cells; IF: 1-2ug/ml
• Immunogen: Recombinant human full-length CD31 protein
• Cellular Localization: Cell Surface and Cytoplasm of endothelial cells
• Positive Control: Jurkat cells. Tonsil or Angiosarcoma.
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C.; Antibody without azide - store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

SDS-Page

(SDS-PAGE Analysis Purified PAX5 Mouse Recombinant Monoclonal Antibody (rPAX5/2060).)

References

Gratzinger D et. al. Am J Clin Pathol 131:264-278 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 313760624
• NCBI GeneID: 5175
• NCBI Accession #: NP_000433.4
• NCBI GenBank Nucleotide #: NM_000442.4
• UniProt Accession #: P16284; Q6LDA9; Q8TBH1; Q96RF5; Q96RF6; Q9NP65; Q9NPB7; Q9NPG9; A0A075B738; A8K3S7; D3DU31
• Molecular Weight: ~100kDa (endothelium); ~130kDa (platelets)
• NCBI Official Full Name: platelet endothelial cell adhesion molecule
• NCBI Official Synonym Full Names: platelet and endothelial cell adhesion molecule 1
• NCBI Official Symbol: PECAM1
• NCBI Official Synonym Symbols: CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM
• NCBI Protein Information: platelet endothelial cell adhesion molecule
• UniProt Protein Name: Platelet endothelial cell adhesion molecule
• UniProt Gene Name: PECAM1
• UniProt Synonym Gene Names: PECAM-1

NCBI Description

The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May 2010]

Uniprot Description

Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (PubMed:19342684, PubMed:17580308). Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (PubMed:19342684). Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions (PubMed:27958302). Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (PubMed:17580308). Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (PubMed:12110892). Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (PubMed:12110892). Modulates bradykinin receptor BDKRB2 activation (PubMed:18672896). Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells (PubMed:18672896). Induces susceptibility to atherosclerosis ().