Mouse CD305 Monoclonal Antibody | anti-CD305 antibody

MOUSE ANTI HUMAN CD305

Cat. Num. AAA212601

• Gene Names: LAIR1; CD305; LAIR-1
• Applications: Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot
• Synonyms: CD305; Monoclonal Antibody; MOUSE ANTI HUMAN CD305; anti-CD305 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: NKTA255
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0 mg/ml (varies by lot)
• Sequence Length: 269

• Applicable Applications for anti-CD305 antibody: Flow cytometry (FC/FACS), Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.; Western Blot: This item detects a band of approximately 40 kDa (1).; Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100
• Perservative Stabilisers: 0.09% Sodium Azide; Preparation
• Immunogen: NK cells lines B12.100 and AM.25; Fusion Partners
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD305)

Testing Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD305 (MBS212602))

Testing Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD305:FITC (MBS210898))

Testing Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD305:RPE (MBS213757))

Related Product Information for anti-CD305 antibody

Mouse anti Human CD305 antibody, clone NKTA255 recognizes human leukocyte-associated Ig-like receptor-1 (LAIR-1), a 40kDa cell surface molecule that functions as an inhibitory receptor on natural killer cells, monocytes and T cells. LAIR-1, otherwise known as CD305, is also expressed by other haematopoeitic cells including B cells, but is not expressed by non-haematopoietic, platelets or erythrocytes. Mouse anti Human CD305 antibody, clone NKTA255 is reported to inhibit NK cell triggering via CD16 molecules (Poggi et al. 1995).

NCBI and Uniprot Product Information

• NCBI GI #: 612407867
• NCBI GeneID: 3903
• NCBI Accession #: NP_001275952.2
• NCBI GenBank Nucleotide #: NM_001289023.2
• UniProt Accession #: Q6GTX8
• Molecular Weight: 23,049 Da
• NCBI Official Full Name: leukocyte-associated immunoglobulin-like receptor 1 isoform c
• NCBI Official Synonym Full Names: leukocyte-associated immunoglobulin-like receptor 1
• NCBI Official Symbol: LAIR1
• NCBI Official Synonym Symbols: CD305; LAIR-1
• NCBI Protein Information: leukocyte-associated immunoglobulin-like receptor 1; leukocyte-associated Ig-like receptor 1
• UniProt Protein Name: Leukocyte-associated immunoglobulin-like receptor 1
• UniProt Gene Name: LAIR1
• UniProt Synonym Gene Names: CD305; LAIR-1; hLAIR1
• UniProt Entry Name: LAIR1_HUMAN

NCBI Description

The protein encoded by this gene is an inhibitory receptor found on peripheral mononuclear cells, including natural killer cells, T cells, and B cells. Inhibitory receptors regulate the immune response to prevent lysis of cells recognized as self. The gene is a member of both the immunoglobulin superfamily and the leukocyte-associated inhibitory receptor family. The gene maps to a region of 19q13.4 called the leukocyte receptor cluster, which contains at least 29 genes encoding leukocyte-expressed receptors of the immunoglobulin superfamily. The encoded protein has been identified as an anchor for tyrosine phosphatase SHP-1, and may induce cell death in myeloid leukemias. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Uniprot Description

LAIR-1: an inhibitory receptor found on peripheral mononuclear cells, including NK cells, T cells, and B cells. Inhibitory receptors regulate the immune response to prevent lysis of cells recognized as self. The gene is a member of both the immunoglobulin superfamily and the leukocyte-associated inhibitory receptor family. The gene maps to a region called the leukocyte receptor cluster, which contains at least 25 genes encoding leukocyte-expressed receptors of the immunoglobulin superfamily.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 19q13.4

Cellular Component: plasma membrane; integral to membrane

Molecular Function: protein binding

Biological Process: immune system process