Mouse anti-Human CD30 Monoclonal Antibody | anti-CD30 antibody

Anti-Hu CD30 Alexa Fluor700

• Gene Names: TNFRSF8; CD30; Ki-1; D1S166E
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: The purified antibody is conjugated with Alexa Fluor700 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Synonyms: CD30; Monoclonal Antibody; Anti-Hu CD30 Alexa Fluor700; Ki-1; TNFRSF8; CD30L receptor; D1S166E; TNF receptor superfamily member 8; anti-CD30 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG
• Clone Number: MEM-268
• Specificity: The antibody MEM-268 recognizes extracellular part of CD30 (Ki-1 antigen), a 105kDa single chain glycoprotein expressed on Hodgkin's and Reed-Sternberg cells; it is also found in Burkitt's lymphomas, virus-infected T and B lymphocytes, and on normal B and T lymphocytes after activation (T lymphocytes that produce Th2-type cytokines and on CD4+/CD8+ T lymphocytes that co-express CD45RO and the IL4 receptor).
• Purity/Purification: The purified antibody is conjugated with Alexa Fluor700 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Form/Format: Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide

• Applicable Applications for anti-CD30 antibody: Flow Cytometry (FC/FACS) (QC tested)
• Application Notes: FC/FACS: The reagent is designed for analysis of human blood cells using 4ul reagent/100ul of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
• Conjugation: Alexa Fluor700
• Immunogen: Expression vector containing CD30 cDNA (booster suspension of THP-1 cell line)
• Preparation and Storage: Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

Flow Cytometry (FC/FACS)

(Flow cytometry surface staining pattern of human PHA stimulated peripheral blood mononuclear cells stained using anti-human CD30 (MEM-268) Alexa Fluor 700 antibody (4 ul reagent per milion cells in 100 ul of cell suspension).)

Flow Cytometry (FC/FACS)

(Separation of human CD30 positive cells (red-filled) from human CD30 negative cells (black-dashed) in flow cytometry analysis (surface staining) of human PHA stimulated peripheral blood mononuclear cells stained using anti-human CD30 (MEM-268) Alexa Fluor 700 antibody (4 ul reagent per milion cells in 100 ul of cell suspension).)

Related Product Information for anti-CD30 antibody

CD30 is a type I transmembrane glycoprotein of the TNF receptor superfamily. CD30 was originally identified as a cell surface antigen of Hodgkins and Reed-Sternberg cells using monoclonal antibody Ki-1. The ligand for CD30 is CD30L (CD153). The binding of CD30 to CD30L mediates pleiotropic effects including cell proliferation, activation, differentiation, and apoptotic cell death. CD30 has a critical role in the pathophysiology of Hodgkin's disease and other CD30+ lymphomas. CD30 acts as a costimulatory molecule in thymic negative selection. In addition to its expression on Hodgkin's and Reed-Sternberg cells, CD30 is also found in some non-Hodgkin's lymphomas (including Burkitt's lymphomas), virus-infected T and B cells, and on normal T and B cells after activation. In T cells, CD30 expression is present on a subset of T cells that produce Th2-type cytokines and on CD4+/CD8+ thymocytes that co-express CD45RO and the IL4 receptor. Soluble form of CD30 (sCD30) serves as a marker reflecting Th2 immune response.

References

*Pavlov I, Martins TB, Delgado JC: Development and validation of a fluorescent microsphere immunoassay for soluble CD30 testing. Clin Vaccine Immunol. 2009 Sep;16(9):1327-31., URL: http://www.ncbi.nlm.nih.gov/pubmed/19605595
*Wright CW, Rumble JM, Duckett CS: CD30 activates both the canonical and alternative NF-kappaB pathways in anaplastic large cell lymphoma cells. J Biol Chem. 2007 Apr;282(14):10252-62., URL: https://www.ncbi.nlm.nih.gov/pubmed/17261581ordin,*Zeiser R, Nguyen VH, Hou JZ, Beilhack A, Zambricki E, Buess M, Contag CH, Negrin RS: Early CD30 signaling is critical for adoptively transferred CD4+CD25+ regulatory T cells in prevention of acute graft-versus-host disease. Blood. 2007 Mar 1;109(5):2225-33., URL: https://www.ncbi.nlm.nih.gov/pubmed/17068147ordin,*Kusanovic JP, Romero R, Hassan SS, Gotsch F, Edwin S, Chaiworapongsa T, Erez O, Mittal P, Mazaki-Tovi S, Soto E, Than NG, Friel LA, Yoon BH, Espinoza J: Maternal serum soluble CD30 is increased in normal pregnancy, but decreased in preeclampsia and small for gestational age pregnancies. J Matern Fetal Neonatal Med. 2007 Aug 28:1-12, URL: https://www.ncbi.nlm.nih.gov/pubmed/17853188ordin,*Bekiaris V, Withers D, Glanville SH, McConnell FM, Parnell SM, Kim MY, Gaspal FM, Jenkinson E, Sweet C, Anderson G, Lane PJ: Role of CD30 in B/T segregation in the spleen. J Immunol. 2007 Dec 1;179(11):7535-43., URL: https://www.ncbi.nlm.nih.gov/pubmed/18025198ordin,*Polte T, Behrendt AK, Hansen G: Direct evidence for a critical role of CD30 in the development of allergic asthma. J Allergy Clin Immunol. 2006 Oct;118(4):942-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/17030250ordin,*Kennedy MK, Willis CR, Armitage RJ: Deciphering CD30 ligand biology and its role in humoral immunity. Immunology. 2006 Jun;118(2):143-52., URL: https://www.ncbi.nlm.nih.gov/pubmed/16771849ordin,*Fischer M, Harvima IT, Carvalho RF, Möller C, Naukkarinen A, Enblad G, Nilsson G: Mast cell CD30 ligand is upregulated in cutaneous inflammation and mediates degranulation-independent chemokine secretion. J Clin Invest. 2006 Oct;116(10):2748-56., URL: https://www.ncbi.nlm.nih.gov/pubmed/16964309ordin,*Blazar BR, Levy RB, Mak TW, Panoskaltsis-Mortari A, Muta H, Jones M, Roskos M, Serody JS, Yagita H, Podack ER, Taylor PA: CD30/CD30 ligand (CD153) interaction regulates CD4+ T cell-mediated graft-versus-host disease. J Immunol. 2004 Sep 1;173(5):2933-41., URL: https://www.ncbi.nlm.nih.gov/pubmed/15322151ordin

NCBI and Uniprot Product Information

• NCBI GI #: 597709795
• NCBI GeneID: 943
• NCBI Accession #: NP_001234.3
• NCBI GenBank Nucleotide #: NM_001243.4
• UniProt Accession #: P28908; Q6P4D9; B1AN79; B9EGD9; D3YTD8
• Molecular Weight: 50,935 Da
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 8 isoform 1
• NCBI Official Synonym Full Names: tumor necrosis factor receptor superfamily member 8
• NCBI Official Symbol: TNFRSF8
• NCBI Official Synonym Symbols: CD30; Ki-1; D1S166E
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 8
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 8
• Protein Family: CD302 antigen
• UniProt Gene Name: TNFRSF8
• UniProt Synonym Gene Names: CD30; D1S166E
• UniProt Entry Name: TNR8_HUMAN

Uniprot Description

TNFRSF8: Receptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF- kappa-B. 2 isoforms of the human protein are produced by alternative initiation.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 1p36

Cellular Component: integral to plasma membrane

Molecular Function: transmembrane receptor activity; tumor necrosis factor receptor activity

Biological Process: immune response; inflammatory response; multicellular organismal development; negative regulation of cell proliferation; positive regulation of apoptosis; positive regulation of TRAIL biosynthetic process; positive regulation of tumor necrosis factor biosynthetic process; response to lipopolysaccharide; signal transduction