Hamster anti-Mouse CD3, epsilon Monoclonal Antibody | anti-CD3E antibody

CD3, epsilon (Early T Cell Marker)

Cat. Num. AAA642708

• Gene Names: CD3E; CD3
• Reactivity: Mouse
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: CD3; epsilon; Monoclonal Antibody; epsilon (Early T Cell Marker); Anti -CD3; anti-CD3E antibody

• Host: Hamster
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 145-2C11
• Specificity: Recognizes mouse CD3 epsilon (CD3e) expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-CD3E antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: Suitable for use in Flow Cytometry, Immunofluorescence, Immunohistochemistry (frozen), Immunoprecipitation, and Western Blot.; Dilution: Flow Cytometry: 1:25-1:200
• Immunogen: H-2Kb-specific mouse cytotoxic T lymphocyte.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-CD3E antibody

CD3 epsilon, a 20kDa transmembrane protein, is a member of the CD3 complex which consists of four subunits, gamma, delta, epsilon and zeta, that are associated to the T cell receptor (TCR). TCR plays a critical role in T cell development and function, and is responsible for ligand recognition. It interacts non-covalently with the CD3 dimers delta/epsilon, gamma/epsilon and zeta/zeta which transduce signals from the TCR into the cell.

Product Categories/Family for anti-CD3E antibody

Antibodies; Abs to CD Markers

NCBI and Uniprot Product Information

• NCBI GI #: 32492880
• NCBI GeneID: 397455
• UniProt Accession #: Q7YRN2
• Molecular Weight: 22,198 Da
• NCBI Official Full Name: CD3 epsilon subunit
• NCBI Official Symbol: CD3E
• NCBI Official Synonym Symbols: CD3
• NCBI Protein Information: T-cell surface glycoprotein CD3 epsilon chain; CD3 epsilon subunit
• UniProt Protein Name: T-cell surface glycoprotein CD3 epsilon chain
• Protein Family: T-cell surface glycoprotein
• UniProt Gene Name: CD3E
• UniProt Entry Name: CD3E_PIG

Uniprot Description

Function: The CD3 complex mediates signal transduction

By similarity.

Subunit structure: The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta

By similarity.

Subcellular location: Membrane; Single-pass type I membrane protein

By similarity.

Sequence similarities: Contains 1 Ig-like (immunoglobulin-like) domain.Contains 1 ITAM domain.