anti-Mouse CD3 epsilon Monoclonal Antibody | anti-cd3e antibody
Hamster Anti-Mouse CD3e-FITC
Reactivity
Mouse
Applications
Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blot
Synonyms
CD3 epsilon; Monoclonal Antibody; Hamster Anti-Mouse CD3e-FITC; Hamster Anti-Mouse CD3e - FITC; Hamster Anti-Mouse CD3 epsilon; anti-cd3e antibody
Host
Armenian Hamster
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Armenian Hamster IgG kappa
Clone Number
145-2C11
Specificity
Mouse CD3 epsilon (Mr 25 kDa)
CD3 epsilon, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chains ranging in size from 16-28 kDa and is closely associated with the T cell antigen receptor (TCR). It is expressed on all T cells of all mouse strains. CD3 plays a major role in signaling during antigen recognition, leading to T-cell activation. The 145-2C11 monoclonal antibody recognizes an epitope on the 25kD epsilon chain of the CD3/TCR complex. In the presence of Fc receptor-bearing accessory cells, soluble 145-2C11 can activate primed and naïve T cell in vitro. 145-2C11 can also induce redirected lysis of specific target cells by CTL clones and it can block lysis of specific target cells by antigen-specific CTL's. Immobilized 145- 2C11 can activate both normal T lymphocytes and cloned T cell lines. Under certain conditions, T cell activation by 145-2C11 may result in apoptotic cell death. ^1-6
CD3 epsilon, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chains ranging in size from 16-28 kDa and is closely associated with the T cell antigen receptor (TCR). It is expressed on all T cells of all mouse strains. CD3 plays a major role in signaling during antigen recognition, leading to T-cell activation. The 145-2C11 monoclonal antibody recognizes an epitope on the 25kD epsilon chain of the CD3/TCR complex. In the presence of Fc receptor-bearing accessory cells, soluble 145-2C11 can activate primed and naïve T cell in vitro. 145-2C11 can also induce redirected lysis of specific target cells by CTL clones and it can block lysis of specific target cells by antigen-specific CTL's. Immobilized 145- 2C11 can activate both normal T lymphocytes and cloned T cell lines. Under certain conditions, T cell activation by 145-2C11 may result in apoptotic cell death. ^1-6
Form/Format
Fluorescein (FITC) Conjugate
Sequence Length
172
Applicable Applications for anti-cd3e antibody
Flow Cytometry; Immunohistochemistry; Immunoprecipitation; Western Blot
Application Notes
Research Application(s): Identification and enumeration of CD3^+ cells by flow cytometry
In vitro depletion of CD3 cells
In vitro activation of T cells-4
Flow Cytometry Suggested Dilution: <= 3 ug/10^6 cells
In vitro depletion of CD3 cells
In vitro activation of T cells-4
Flow Cytometry Suggested Dilution: <= 3 ug/10^6 cells
Immunogen
H-2K^b-specific mouse cytotoxic T lymphocyte clone BM10-37
Characterization
To insure acceptable performance, each batch of product is tested by flow cytometry to conform to the characteristics of a standard reference. Representative data are included in this product insert.
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The fluorescein (FITC) and Cyanine 5 (CY 5) conjugates are supplied as 0.5 mg or 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE), allophycocyanin (APC), and Spectral Red (SPRD) conjugates are supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
The Alexa Fluor 647 (AF647) conjugate is supplies as 0.1 mg in 0.2mL of PBS/NaN3. Store at 2-8 degree C.
Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
The fluorescein (FITC) and Cyanine 5 (CY 5) conjugates are supplied as 0.5 mg or 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE), allophycocyanin (APC), and Spectral Red (SPRD) conjugates are supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
The Alexa Fluor 647 (AF647) conjugate is supplies as 0.1 mg in 0.2mL of PBS/NaN3. Store at 2-8 degree C.
Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
References
1. Leo, O., M. Foo, D. Sachs, L. Samuelson, and J. Bluestone. 1987. Proc. Natl. Acad. Sci. USA 84:1374.
2. Portoles, P., J. Rojo, A. Golby, M. Bonneville, S. Gromkowski, L. Greenbaum, C. A. Janeway, Jr., D. B. Murphy, and K. Bottomly. 1989. J. Immunol. 142:4169.
3. Kruisbeek, A.M., and E. Shevach. 1991. In: Currents Protocols in Immunology. J. Coligan, A. Kruisbeek, D. Margulies, E.M. Shevach, and W. Strober, eds. John Wiley & Sons, New York, pp. 3.12.1-3.12.14.
4. Duke, R.C., J.J. Cohen, S.A. Boehme, M.J. Lenardo, C.D. Surh, H. Kishimoto, and J. Sprent. 1995. In: Currents Protocols in Immunology. J. Coligan, A. Kruisbeek, D. Margulies, E.M. Shevach, and W. Strober, eds. John Wiley & Sons, New York, pp. 3.17.1-3.17.33.
5. Ucker, D.S., J. Meyers, and P.D. Obermiller. 1992. J. Immunol. 149:1583.
6. Wang, R., K.M. Murphy, D.Y. Loh, C. Weaver, and J.H. Russell. 1993. J. Immunol. 150:3832.
7. Salvadori, S., B. Gansbacher, A.M. Pizzimenti, and K.S. Zier. 1994. J. Immunol. 153.5182.
8. Payer, E., A. Elbe, and G. Stingl., 1991. J. Immunol. 146:2536.
2. Portoles, P., J. Rojo, A. Golby, M. Bonneville, S. Gromkowski, L. Greenbaum, C. A. Janeway, Jr., D. B. Murphy, and K. Bottomly. 1989. J. Immunol. 142:4169.
3. Kruisbeek, A.M., and E. Shevach. 1991. In: Currents Protocols in Immunology. J. Coligan, A. Kruisbeek, D. Margulies, E.M. Shevach, and W. Strober, eds. John Wiley & Sons, New York, pp. 3.12.1-3.12.14.
4. Duke, R.C., J.J. Cohen, S.A. Boehme, M.J. Lenardo, C.D. Surh, H. Kishimoto, and J. Sprent. 1995. In: Currents Protocols in Immunology. J. Coligan, A. Kruisbeek, D. Margulies, E.M. Shevach, and W. Strober, eds. John Wiley & Sons, New York, pp. 3.17.1-3.17.33.
5. Ucker, D.S., J. Meyers, and P.D. Obermiller. 1992. J. Immunol. 149:1583.
6. Wang, R., K.M. Murphy, D.Y. Loh, C. Weaver, and J.H. Russell. 1993. J. Immunol. 150:3832.
7. Salvadori, S., B. Gansbacher, A.M. Pizzimenti, and K.S. Zier. 1994. J. Immunol. 153.5182.
8. Payer, E., A. Elbe, and G. Stingl., 1991. J. Immunol. 146:2536.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
CD3 epsilon
NCBI Official Symbol
cd3e
NCBI Protein Information
CD3 epsilon
Protein Family
Similar Products
Product Notes
The cd3e (Catalog #AAA673130) is an Antibody produced from Armenian Hamster and is intended for research purposes only. The product is available for immediate purchase. The Hamster Anti-Mouse CD3e-FITC reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD3 epsilon can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry; Immunohistochemistry; Immunoprecipitation; Western Blot. Research Application(s): Identification and enumeration of CD3^+ cells by flow cytometry In vitro depletion of CD3 cells In vitro activation of T cells-4 Flow Cytometry Suggested Dilution: <= 3 ug/10^6 cells. Researchers should empirically determine the suitability of the cd3e for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD3 epsilon, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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