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Western Blot (WB) (Western blot analysis of extracts of various cell lines, using TUBA4A antibody.)

Rabbit TUBA4A Polyclonal Antibody | anti-TUBA4A antibody

TUBA4A (Tubulin alpha 4A Chain, Tubulin, alpha 4a, TUBA1, Tubulin alpha 1 Chain, FLJ30169, alpha-Tubulin) (PE)

Gene Names
TUBA4A; TUBA1; H2-ALPHA
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Purified by affinity chromatography.
Synonyms
TUBA4A; Polyclonal Antibody; TUBA4A (Tubulin alpha 4A Chain; Tubulin; alpha 4a; TUBA1; Tubulin alpha 1 Chain; FLJ30169; alpha-Tubulin) (PE); anti-TUBA4A antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes TUBA4A. Species Crossreactivity: human, mouse, rat
Purity/Purification
Purified by affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Concentration
~1mg/ml (varies by lot)
Applicable Applications for anti-TUBA4A antibody
Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
IHC: 1:50-1:200
WB: 1:500-1:2000
Applications are based on unconjugated antibody.
Immunogen
Recombinant protein corresponding to human TUBA4A.
Conjugate
PE
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using TUBA4A antibody.)

Western Blot (WB) (Western blot analysis of extracts of various cell lines, using TUBA4A antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human liver cancer using TUBA4A antibody at dilution of 1:200 (200x lens).)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human liver cancer using TUBA4A antibody at dilution of 1:200 (200x lens).)
Product Categories/Family for anti-TUBA4A antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
448
NCBI Official Full Name
Tubulin alpha-4A chain
NCBI Official Synonym Full Names
tubulin, alpha 4a
NCBI Official Symbol
TUBA4A
NCBI Official Synonym Symbols
TUBA1; H2-ALPHA
NCBI Protein Information
tubulin alpha-4A chain; tubulin H2-alpha; tubulin alpha-1 chain; tubulin, alpha 1 (testis specific)
UniProt Protein Name
Tubulin alpha-4A chain
Protein Family
UniProt Gene Name
TUBA4A
UniProt Synonym Gene Names
TUBA1
UniProt Entry Name
TBA4A_HUMAN

NCBI Description

Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2013]

Uniprot Description

TUBA4A: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. Dimer of alpha and beta chains. Belongs to the tubulin family.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: microtubule; extracellular region; cytosol

Molecular Function: GTPase activity; protein binding; GTP binding; structural constituent of cytoskeleton

Biological Process: protein polymerization; 'de novo' posttranslational protein folding; platelet activation; cellular protein metabolic process; platelet degranulation; protein folding; organelle organization and biogenesis; mitotic cell cycle; G2/M transition of mitotic cell cycle; blood coagulation; microtubule-based process

Disease: Amyotrophic Lateral Sclerosis 22 With Or Without Frontotemporal Dementia

Research Articles on TUBA4A

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Product Notes

The TUBA4A tuba4a (Catalog #AAA6441961) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TUBA4A (Tubulin alpha 4A Chain, Tubulin, alpha 4a, TUBA1, Tubulin alpha 1 Chain, FLJ30169, alpha-Tubulin) (PE) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TUBA4A can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Western Blot (WB). IHC: 1:50-1:200 WB: 1:500-1:2000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the TUBA4A tuba4a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TUBA4A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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