Mouse CD3 Monoclonal Antibody | anti-CD3 antibody
MOUSE ANTI MONKEY CD3:FITC
Reactivity
Rhesus Monkey
Applications
Flow Cytometry, Functional Assay
Synonyms
CD3; Monoclonal Antibody; MOUSE ANTI MONKEY CD3:FITC; anti-CD3 antibody
Host
Mouse
Reactivity
Rhesus Monkey
Clonality
Monoclonal
Isotype
IgG1
Clone Number
FN-18
Specificity
CD3
This item recognises the rhesus monkey RhT3 antigen, the homologue of human CD3. Clone FN18 has been reported to recognise a polymorphic epitope of the RhT3 antigen, which is expressed by mature T lymphocytes.
This item recognises the rhesus monkey RhT3 antigen, the homologue of human CD3. Clone FN18 has been reported to recognise a polymorphic epitope of the RhT3 antigen, which is expressed by mature T lymphocytes.
Form/Format
FITC (Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - lyophilised)
Applicable Applications for anti-CD3 antibody
Flow Cytometry (FACS)
Application Notes
Flow Cytometry: Max Dilution: 1/10
Flow Cytometry: Use 50ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Use 50ul of the suggested working dilution to label 106 cells in 100ul.
Immunogen
Rhesus monkey peripheral blood leucocytes
Fusion Partners
Spleen cells from immunised mice were fused with cells of the mouse SP2/0 Ag14 myeloma cell line.
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Preservative Stabilizers
1% Bovine Serum Albumin
125mM Trehalose
125mM Trehalose
Reconstitution
Reconstitute with 0.5ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. We recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide and 1% bovine serum albumin is recommended.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. We recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide and 1% bovine serum albumin is recommended.
Buffer Solution
Phosphate buffered saline
Preparation and Storage
Prior to reconstitution store at +4 degree C. Following reconstitution store at -20 degree C.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from reconstitution
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from reconstitution
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Product Notes
The CD3 (Catalog #AAA210703) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MOUSE ANTI MONKEY CD3:FITC reacts with Rhesus Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's CD3 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FACS). Flow Cytometry: Max Dilution: 1/10 Flow Cytometry: Use 50ul of the suggested working dilution to label 106 cells in 100ul. Researchers should empirically determine the suitability of the CD3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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