Testing Data
(Staining of paraffin embedded rat spleen with Mouse anti Rat CD3)
Mouse CD3 Monoclonal Antibody | anti-CD3 antibody
MOUSE ANTI RAT CD3
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation, Immunohistochemistry
Synonyms
CD3; Monoclonal Antibody; MOUSE ANTI RAT CD3; anti-CD3 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgM
Clone Number
1F4
Form/Format
Purified
Purified IgM - liquid
Purified IgM - liquid
Concentration
IgM concentration 1.0 mg/ml (varies by lot)
Sequence Length
173
Applicable Applications for anti-CD3 antibody
Immunohistology Frozen, Flow cytometry (FC/FACS), Immunoprecipitation (IP), Immunohistology Paraffin*
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Immunohistology - Frozen: Minimum Dilution: 1/10; Maximum Dilution: 1/25
Flow Cytometry: Minimum Dilution: 1/10; Maximum Dilution: 1/25
Immunohistology - Paraffin: Maximum Dilution: 1/10; Application Note: This clone is suitable for use on paraffin embedded material using target unmasking fluid
Immunohistology - Frozen: Minimum Dilution: 1/10; Maximum Dilution: 1/25
Flow Cytometry: Minimum Dilution: 1/10; Maximum Dilution: 1/25
Immunohistology - Paraffin: Maximum Dilution: 1/10; Application Note: This clone is suitable for use on paraffin embedded material using target unmasking fluid
Perservative Stabilisers
0.09% Sodium Azide
Preparation
Preparation
Buffer Solution
Phosphate buffered saline
Target Species
Rat
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Testing Data
(Staining of paraffin embedded rat spleen with Mouse anti Rat CD3)
Testing Data
(Staining of paraffin embedded rat spleen with Mouse anti Rat CD3)
Related Product Information for anti-CD3 antibody
Mouse anti Rat CD3 antibody, clone 1F4 recognizes rat CD3, a 25kD antigen which is found on rat T-cells. Mouse anti Rat CD3, clone 1F4 does not react with rat B cells. In immunohistology it stains rat thymus tissues strongly in the medulla and weakly in the cortex. Functionally the addition of the antibody to a culture of rat T cells induces the proliferation of T-cells in the presence of PMA.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
19,356 Da
NCBI Official Full Name
T-cell surface glycoprotein CD3 delta chain
NCBI Official Synonym Full Names
CD3 molecule, delta
NCBI Official Symbol
Cd3d
NCBI Protein Information
T-cell surface glycoprotein CD3 delta chain; CD3 antigen delta polypeptide; CD3 molecule delta polypeptide; T-cell receptor T3 delta chain
UniProt Protein Name
T-cell surface glycoprotein CD3 delta chain
Protein Family
UniProt Gene Name
Cd3d
UniProt Synonym Gene Names
T3d
UniProt Entry Name
CD3D_RAT
NCBI Description
putative component of the CD3 T-cell receptor [RGD, Feb 2006]
Uniprot Description
The CD3 complex mediates signal transduction.
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Product Notes
The CD3 cd3d (Catalog #AAA213151) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD3 can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen, Flow cytometry (FC/FACS), Immunoprecipitation (IP), Immunohistology Paraffin*. Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Immunohistology - Frozen: Minimum Dilution: 1/10; Maximum Dilution: 1/25 Flow Cytometry: Minimum Dilution: 1/10; Maximum Dilution: 1/25 Immunohistology - Paraffin: Maximum Dilution: 1/10; Application Note: This clone is suitable for use on paraffin embedded material using target unmasking fluid. Researchers should empirically determine the suitability of the CD3 cd3d for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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