Canine Coagulation Factor VIII (F8) ELISA Kit | F8 elisa kit

Canine Coagulation Factor VIII (F8) ELISA Kit

Cat. Num. AAA2019021

• Gene Names: F8; Cf8; Cf-8; FVIII
• Reactivity: Canine
• Synonyms: Coagulation Factor VIII (F8); Canine Coagulation Factor VIII (F8) ELISA Kit; FVIII; AHF; HEMA; F8B; F8C; Antihemophilic Globulin; Hemophilia A; Anti-Hemophilic Factor A; FVIII Procoagulant Component; F8 Protein; F8 elisa kit

• Reactivity: Canine
• Specificity: This assay has high sensitivity and excellent specificity for detection of Coagulation Factor VIII (F8).; No significant cross-reactivity or interference between Coagulation Factor VIII (F8) and analogues was observed.
• Sequence Length: 2319

• Assay Type: Double-antibody Sandwich
• Samples: Plasma
• Detection Range: 78-5000pg/mL
• Sensitivity: < 28pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Coagulation Factor VIII (F8) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Coagulation Factor VIII (F8) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for F8 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Coagulation Factor VIII (F8). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Coagulation Factor VIII (F8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Coagulation Factor VIII (F8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Coagulation Factor VIII (F8) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for F8 elisa kit

Metabolic pathway; Hematology

NCBI and Uniprot Product Information

• NCBI GI #: 238624180
• NCBI GeneID: 14069
• NCBI Accession #: NP_032003.2
• NCBI GenBank Nucleotide #: NM_007977.2
• UniProt Accession #: Q06194; A2AN88
• Molecular Weight: 266,196 Da
• NCBI Official Full Name: coagulation factor VIII isoform 1
• NCBI Official Synonym Full Names: coagulation factor VIII
• NCBI Official Symbol: F8
• NCBI Official Synonym Symbols: Cf8; Cf-8; FVIII
• NCBI Protein Information: coagulation factor VIII
• UniProt Protein Name: Coagulation factor VIII
• Protein Family: Coagulation factor
• UniProt Gene Name: F8
• UniProt Synonym Gene Names: Cf8; F8c
• UniProt Entry Name: FA8_MOUSE

Uniprot Description

F8: Factor VIII, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa. Defects in F8 are the cause of hemophilia A (HEMA). A disorder of blood coagulation characterized by a permanent tendency to hemorrhage. About 50% of patients have severe hemophilia resulting in frequent spontaneous bleeding into joints, muscles and internal organs. Less severe forms are characterized by bleeding after trauma or surgery. Of particular interest for the understanding of the function of F8 is the category of CRM (cross-reacting material) positive patients (approximately 5%) that have considerable amount of F8 in their plasma (at least 30% of normal), but the protein is non- functional; i.e. the F8 activity is much less than the plasma protein level. CRM-reduced is another category of patients in which the F8C antigen and activity are reduced to approximately the same level. Most mutations are CRM negative, and probably affect the folding and stability of the protein. Belongs to the multicopper oxidase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: extracellular space; extracellular region

Molecular Function: copper ion binding; metal ion binding; serine-type endopeptidase activity; oxidoreductase activity

Biological Process: platelet activation; hemostasis; acute-phase response; proteolysis; blood coagulation; blood coagulation, intrinsic pathway