WB (Western Blot)
(Detection of human MARS by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, HeLa, and Jurkat cells. Antibodies: Affinity purified rabbit anti-MARS antibody AAA212409 (lot AAA212409-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human MARS Polyclonal Antibody | anti-MARS antibody
Rabbit anti-MARS Antibody, Affinity Purified
Gene Names
MARS; MRS; METRS; MTRNS
Reactivity
Human
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
MARS, Antibody; Rabbit anti-MARS Antibody, Affinity Purified; cytosolic methionyl-tRNA synthetase; SPG70; MTRNS; MRS; METRS; Methionyl-tRNA synthetase; methionine--tRNA ligase, cytoplasmic; ILFS2; CMT2U; ILLD; Methionine--tRNA ligase, cytoplasmic; methionyl-tRNA synthetase; anti-MARS antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-MARS antibody
IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 200 and 250
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human MARS by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, HeLa, and Jurkat cells. Antibodies: Affinity purified rabbit anti-MARS antibody AAA212409 (lot AAA212409-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human MARS by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, HeLa, and Jurkat cells. Antibodies: Affinity purified rabbit anti-MARS antibody AAA212409 (lot AAA212409-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human MARS by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-MARS antibody AAA212409 (lot AAA212409-1) used for IP at 6 ug/mg lysate. MARS was also immunoprecipitated by rabbit anti-MARS antibody For blotting immunoprecipitated MARS, AAA212409 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation)
(Detection of human MARS by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-MARS antibody AAA212409 (lot AAA212409-1) used for IP at 6 ug/mg lysate. MARS was also immunoprecipitated by rabbit anti-MARS antibody For blotting immunoprecipitated MARS, AAA212409 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IHC (Immunohistochemistry)
(Detection of human MARS by immunohistochemistry. Samples: FFPE serial sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-MARS (Cat. No. AAA212409 Lot1, left image and Cat. No. Lot1, right image) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human MARS by immunohistochemistry. Samples: FFPE serial sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-MARS (Cat. No. AAA212409 Lot1, left image and Cat. No. Lot1, right image) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)
Related Product Information for anti-MARS antibody
Methionyl-tRNA synthetase (MARS) is a member of the class I family of aminoacyl-tRNA synthetases. These enzymes play a critical role in protein biosynthesis by charging tRNAs with their cognate amino acids. The encoded protein is a component of the multi-tRNA synthetase complex and catalyzes the ligation of methionine to tRNA molecules. [taken from NCBI Entrez Gene (Gene ID: 4141)].
Product Categories/Family for anti-MARS antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
101,116 Da
NCBI Official Full Name
methionine--tRNA ligase, cytoplasmic
NCBI Official Synonym Full Names
methionyl-tRNA synthetase
NCBI Official Symbol
MARS
NCBI Official Synonym Symbols
MRS; METRS; MTRNS
NCBI Protein Information
methionine--tRNA ligase, cytoplasmic; cytosolic methionyl-tRNA synthetase; methionine tRNA ligase 1, cytoplasmic
UniProt Protein Name
Methionine--tRNA ligase, cytoplasmic
UniProt Gene Name
MARS
UniProt Synonym Gene Names
MetRS
UniProt Entry Name
SYMC_HUMAN
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Product Notes
The MARS mars (Catalog #AAA212409) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-MARS Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MARS can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the MARS mars for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MARS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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