Mouse anti-Human CD28 Monoclonal Antibody | anti-CD28 antibody

CD28 FITC Monoclonal Antibody (Clone CD28.2)

Cat. Num. AAA840123

• Gene Names: CD28; Tp44
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: Affinity chromatography.
• Synonyms: CD28; Monoclonal Antibody; CD28 FITC Monoclonal Antibody (Clone CD28.2); anti-CD28 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1, Kappa
• Clone Number: CD28.2
• Purity/Purification: Affinity chromatography.
• Form/Format: Phosphate-buffered aqueous solution pH 7.2, <=0.09% Sodium azide, may contain carrier protein/stabilizer.; Appearance: Colorless liquid
• Sequence Length: 123

• Applicable Applications for anti-CD28 antibody: Flow Cytometry (FC/FACS)
• Application Notes: Flow (Cell Surface): 5 ul/1x10^6 cells, Volume per test: 5 ul (0.5 ug).
• Immunogen: Human CD28
• Positive Control: FACS: Human peripheral blood lymphocytes
• Handling: The antibody solution should be gently mixed before use.
• Preparation and Storage: 4 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for anti-CD28 antibody

Mouse monoclonal FITC conjugated antibody to detect CD28 in human samples.

Background: CD28 (Cluster of Differentiation 28) is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2). When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells. The CD28.2 monoclonal antibody specifically binds with the human 44 kDa homodimeric trans-membrane glycoprotein CD28, expressed on the surface of most mature T lymphocytes, plasma cells, and thymocytes. CD28 is a ligand for B7-1 (CD80) and B7-2 (CD86), a co-stimulator of T lymphocytes, and enhances the interaction between T and B lymphocytes. It has been reported that the T lymphocytes stimulation to produce IL-2 depends on the monoclonal antibody involved, which suggests that the CD28 molecule presents some sub regions with distinct functions. The CD28.2 antibody induces Ca2+ influx in Jurkat T lymphocytes. Other studies have shown that CD28 is involved in the signal transduction.

Product Categories/Family for anti-CD28 antibody

Antibodies & Supporting Tools; FACS Antibodies (New) Antibodies & Supporting Tools; Primary Antibodies (A-Z)

NCBI and Uniprot Product Information

• NCBI GI #: 340545508
• NCBI GeneID: 940
• NCBI Accession #: NP_001230006.1
• NCBI GenBank Nucleotide #: NM_001243077.1
• UniProt Accession #: P10747; Q13964; Q52M23; Q70WG0; Q8NI54; Q8NI55; Q8NI56; Q8WXJ2; Q9BYV0; A8KAC1
• Molecular Weight: 26,186 Da
• NCBI Official Full Name: T-cell-specific surface glycoprotein CD28 isoform 2
• NCBI Official Synonym Full Names: CD28 molecule
• NCBI Official Symbol: CD28
• NCBI Official Synonym Symbols: Tp44
• NCBI Protein Information: T-cell-specific surface glycoprotein CD28
• UniProt Protein Name: T-cell-specific surface glycoprotein CD28
• Protein Family: T-cell-specific surface glycoprotein
• UniProt Gene Name: CD28
• UniProt Entry Name: CD28_HUMAN

NCBI Description

The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]

Uniprot Description

CD28: a type I plasma membrane protein Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Binds to B7-1 and -2. Expressed in T cells and plasma cells, but not in less mature B cells. Six splice-variant isoforms have been described.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 2q33

Cellular Component: integral to plasma membrane; plasma membrane; immunological synapse; cytosol; external side of plasma membrane

Molecular Function: identical protein binding; protein binding; protease binding; SH3/SH2 adaptor activity; coreceptor activity

Biological Process: positive regulation of isotype switching to IgG isotypes; viral reproduction; positive regulation of translation; nerve growth factor receptor signaling pathway; positive regulation of interleukin-2 biosynthetic process; cytokine biosynthetic process; T cell receptor signaling pathway; positive regulation of inflammatory response to antigenic stimulus; cell surface receptor linked signal transduction; positive regulation of T cell proliferation; regulatory T cell differentiation; epidermal growth factor receptor signaling pathway; positive regulation of viral genome replication; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; positive regulation of mitosis; negative thymic T cell selection; regulation of defense response to virus by virus; humoral immune response; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; T cell costimulation; innate immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of alpha-beta T cell proliferation