Testing Data
Mouse anti-Human CD45RA Monoclonal Antibody | anti-CD45RA antibody
CD45RA FITC Monoclonal Antibody (Clone HI100)
Appearance: Colorless liquid
Shelf Life: 12 months
Testing Data
Testing Data
Background: CD45, a transmembrane multifunctional glycoprotein, is a member of Type I receptor-linked PTPase family and is expressed as multiple isoforms due to alternative splicing. Expression of these isoforms is highly regulated and shift in this expression determines T-cell activation. CD45RB consists of exon B and is predominantly expressed in naïve T-cells secreting IL-2. Its expression is low in primed/memory T cells, cells that express Th2 cytokines such as IL-4 and IL-10 and population of T-cells with regulatory function. Immunotherapy with CD45RB antibody is being widely studied in transplantation and vaccination. CD45 antibodies are commonly used to identify tumors of lymphoid origin. The HI100 monoclonal antibody specifically reacts with human CD45RA, the 220 kDa isoform of the human leukocyte common antigen (LCA) found on 40-50% of the peripheral CD4+ T lymphocytes, half of the peripheral CD8+ T lymphocytes and some of the monocytes and B lymphocytes. The CD45RA antigen is expressed by naïve and activated T lymphocytes. The HI100 monoclonal antibody is used as a phenotypic marker to discriminate T lymphocytes subsets.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jun 2012]
Uniprot Description
CD45: a receptor type protein tyrosine phosphatase protein. Contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. The first PTPAse domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Specifically expressed in hematopoietic cells. An essential regulator of T- and B-cell antigen receptor signaling. Functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. Suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced isoforms have been described.
Protein type: Receptor protein phosphatase, tyrosine; EC 3.1.3.48; Membrane protein, integral; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 1q31-q32
Cellular Component: focal adhesion; membrane; integral to plasma membrane; plasma membrane; lipid raft; external side of plasma membrane
Molecular Function: protein binding; transmembrane receptor protein tyrosine phosphatase activity; protein tyrosine phosphatase activity; protein kinase binding
Biological Process: B cell proliferation; axon guidance; substrate-bound cell migration, cell release from substrate; regulation of cell cycle; protein amino acid dephosphorylation; T cell receptor signaling pathway; positive regulation of antigen receptor-mediated signaling pathway; bone marrow development; stem cell development; B cell receptor signaling pathway; cell surface receptor linked signal transduction; dephosphorylation; release of sequestered calcium ion into cytosol; positive regulation of protein kinase activity; negative regulation of T cell mediated cytotoxicity; positive regulation of B cell proliferation; negative regulation of protein kinase activity; negative regulation of cytokine and chemokine mediated signaling pathway; hemopoietic progenitor cell differentiation; positive regulation of T cell proliferation; immunoglobulin biosynthetic process; defense response to virus; T cell differentiation
Disease: Severe Combined Immunodeficiency, Autosomal Recessive, T Cell-negative, B Cell-positive, Nk Cell-positive; Hepatitis C Virus, Susceptibility To
Research Articles on CD45RA
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Product Notes
The CD45RA ptprc (Catalog #AAA840008) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CD45RA FITC Monoclonal Antibody (Clone HI100) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD45RA can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). Flow (Cell Surface): 5 ul/1x10^6 cells, Volume per test: 5 ul (1 ug). Researchers should empirically determine the suitability of the CD45RA ptprc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD45RA, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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