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Flow Cytometry (FC/FACS) (Separation of human CD297 positive cells (red-filled) from cellular debris (black-dashed) in flow cytometry analysis (surface staining) of human PHA stimulated peripheral blood mononuklear cells stained using anti-human CD279 (EH12.2H7) APC antibody (10 ul reagent per milion cells in 100 ul of cell suspension).)

Mouse CD279 Monoclonal Antibody | anti-CD279 antibody

Anti-Hu CD279 APC

Gene Names
PDCD1; PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1
Reactivity
Non-human primates, Human
Applications
Flow Cytometry, Functional Assay
Purity
The purified antibody is conjugated with allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Synonyms
CD279; Monoclonal Antibody; Anti-Hu CD279 APC; programmed cell death 1; PDCD1; PD1; PD-1; SLEB2; anti-CD279 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Non-human primates, Human
Clonality
Monoclonal
Isotype
IgG1 kappa
Clone Number
EH12.2H7
Specificity
The mouse monoclonal antibody EH12.2H7 recognizes an extracellular epitope of CD279 / PD-1 (programmed cell death 1), a 55 kDa type I transmembrane protein expressed above all during T cell development, on activated T cells, activated B cells, and activated monocytes.
Purity/Purification
The purified antibody is conjugated with allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Form/Format
Stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide
Sequence Length
288
Applicable Applications for anti-CD279 antibody
Flow Cytometry (FC/FACS)
Application Notes
FC/FACS: QC tested
Conjugation
APC
Preparation and Storage
Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light.

Flow Cytometry (FC/FACS)

(Separation of human CD297 positive cells (red-filled) from cellular debris (black-dashed) in flow cytometry analysis (surface staining) of human PHA stimulated peripheral blood mononuklear cells stained using anti-human CD279 (EH12.2H7) APC antibody (10 ul reagent per milion cells in 100 ul of cell suspension).)

Flow Cytometry (FC/FACS) (Separation of human CD297 positive cells (red-filled) from cellular debris (black-dashed) in flow cytometry analysis (surface staining) of human PHA stimulated peripheral blood mononuklear cells stained using anti-human CD279 (EH12.2H7) APC antibody (10 ul reagent per milion cells in 100 ul of cell suspension).)
Related Product Information for anti-CD279 antibody
CD279 / PD-1 (programmed cell death 1), a transmembrane protein of CD28/CTLA-4 family. It is expressed inducibly mainly on activated T, B, and myeloid cells and plays a role in maintaining peripheral self-tolerance. Binding to its receptors CD273 and CD274 is associated with inhibition of T cell proliferation and induction of their anergy. It is also expressed during thymic development. Some variants of CD279 are associated with susceptibility to systemic lupus erythematosus, type 1 diabetes, and rheumatoid arthritis.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Programmed cell death protein 1
NCBI Official Synonym Full Names
programmed cell death 1
NCBI Official Symbol
PDCD1
NCBI Official Synonym Symbols
PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1
NCBI Protein Information
programmed cell death protein 1
UniProt Protein Name
Programmed cell death protein 1
UniProt Gene Name
PDCD1
UniProt Synonym Gene Names
PD1; Protein PD-1; hPD-1

NCBI Description

This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases. [provided by RefSeq, Jul 2008]

Uniprot Description

Inhibitory cell surface receptor involved in the regulation of T-cell function during immunity and tolerance. Upon ligand binding, inhibits T-cell effector functions in an antigen-specific manner. Possible cell death inducer, in association with other factors.

Research Articles on CD279

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Product Notes

The CD279 pdcd1 (Catalog #AAA1800576) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Hu CD279 APC reacts with Non-human primates, Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD279 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC/FACS: QC tested. Researchers should empirically determine the suitability of the CD279 pdcd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD279, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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