Rabbit anti-Human CHEK1 Polyclonal Antibody | anti-CHEK1 antibody

CHEK1, Phosphorylated (S317) (Cell Cycle Checkpoint Kinase, CHK1) (PE)

Cat. Num. AAA6411524

• Gene Names: CHEK1; CHK1
• Reactivity: Human
• Applications: Immunofluorescence, Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: CHEK1; Polyclonal Antibody; Phosphorylated (S317) (Cell Cycle Checkpoint Kinase; CHK1) (PE); anti-CHEK1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CHEK1, phosphorylated S317.
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-CHEK1 antibody: Immunofluorescence (IF), Western Blot (WB)
• Application Notes: IF: 1:50-1:200; WB: 1:500-1:2000; Applications are based on unconjugated antibody.
• Immunogen: Phospho specific peptide corresponding to residues surrounding S317 of human CHEK1.
• Conjugate: PE
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of MCF7 cell line, using Phospho-CHEK1-S317 antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of MCF7 cell using Phospho-CHEK1-S317 antibody. Blue: DAPI for nuclear staining.)

Product Categories/Family for anti-CHEK1 antibody

Antibodies; Cancer Markers

NCBI and Uniprot Product Information

• NCBI GI #: 166295194
• NCBI GeneID: 1111
• NCBI Accession #: NP_001107593.1
• NCBI GenBank Nucleotide #: NM_001114121.2
• UniProt Accession #: O14757; A8K934; B4DDD0; B4DSK3; B5BTY6; F5H7S4; H2BI51
• Molecular Weight: 54,434 Da
• NCBI Official Full Name: serine/threonine-protein kinase Chk1 isoform 1
• NCBI Official Synonym Full Names: checkpoint kinase 1
• NCBI Official Symbol: CHEK1
• NCBI Official Synonym Symbols: CHK1
• NCBI Protein Information: serine/threonine-protein kinase Chk1; Chk1-S; checkpoint kinase-1; CHK1 checkpoint homolog; cell cycle checkpoint kinase; Checkpoint, S. pombe, homolog of, 1
• UniProt Protein Name: Serine/threonine-protein kinase Chk1
• UniProt Gene Name: CHEK1
• UniProt Synonym Gene Names: CHK1
• UniProt Entry Name: CHK1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

Chk1: a protein kinase of the CAMKL family. Required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. Plays an important role in both S and G2 checkpoints, embryonic development and tumor suppression. Phosphorylated and activated by ATM/ATR following DNA damage, it binds to single-stranded DNA and DNA ends and has a role in the repair of double strand breaks. Activated Chk1 can phosphorylate and inactivate cdc25C via phosphorylation and 14-3-3 binding, blocking the activation of cdc2 and transition into M-phase. Other substrates include p53. Implicated in resistance to apoptosis in response to chemotherapy. Inhibitors under development to chemosensitize tumors. Somatic mutations found in stomach tumors, and in colon and endometrial tumors, where CHK1 may be a target of microsatellite instability. Inhibitors: SB218078, UNC-01.

Protein type: Tumor suppressor; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CAMK; Kinase, protein; EC 2.7.11.1; CAMK group; CAMKL family; CHK1 subfamily

Chromosomal Location of Human Ortholog: 11q24.2

Cellular Component: nucleoplasm; centrosome; extracellular space; chromosome, telomeric region; condensed nuclear chromosome; intracellular membrane-bound organelle; replication fork; nucleus; chromatin; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding

Biological Process: DNA damage induced protein phosphorylation; chromatin-mediated maintenance of transcription; regulation of mitotic centrosome separation; negative regulation of mitosis; DNA damage checkpoint; peptidyl-threonine phosphorylation; G2/M transition of mitotic cell cycle; G2/M transition DNA damage checkpoint; DNA repair; DNA replication; response to DNA damage stimulus; regulation of cell proliferation