Flow Cytometry (FC/FACS)
(Staining of BALB/c splenocytes with CD45R antibody (FITC) and 0.125 ug CD27 antibody followed by Anti-Armenian Hamster IgG (PE). Total viable cells were analyzed.)
Hamster anti-Mouse CD27 Monoclonal Antibody | anti-CD27 antibody
CD27 antibody
Gene Names
CD27; T14; S152; Tp55; TNFRSF7; S152. LPFS2
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Immunoprecipitation
Purity
Purified by affinity chromatography.
Synonyms
CD27; Monoclonal Antibody; CD27 antibody; Armenian Hamster monoclonal CD27 antibody; Monoclonal CD27 antibody; Anti-CD27 antibody; CD 27; TNFRSF7 antibody; CD-27; T14 antibody; CD 27 antibody; CD-27 antibody; anti-CD27 antibody
Host
Hamster
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG
Clone Number
LG.3A10
Specificity
Mouse
Purity/Purification
Purified by affinity chromatography.
Form/Format
Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration
500 ug/ml (varies by lot)
Sequence Length
260
Applicable Applications for anti-CD27 antibody
Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Application Notes
FC/FACS: = 0.125 ug/test
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest.
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest.
Cross-Reactivity
Human, Rat
Preparation and Storage
Store at 2-8 degree C. Do not freeze. Protect from light.
Flow Cytometry (FC/FACS)
(Staining of BALB/c splenocytes with CD45R antibody (FITC) and 0.125 ug CD27 antibody followed by Anti-Armenian Hamster IgG (PE). Total viable cells were analyzed.)
Flow Cytometry (FC/FACS)
(Staining of BALB/c splenocytes with CD45R antibody (FITC) and 0.125 ug CD27 antibody followed by Anti-Armenian Hamster IgG (PE). Total viable cells were analyzed.)
Related Product Information for anti-CD27 antibody
The LG.3A10 monoclonal antibody reacts with mouse CD27, a lymphocyte-specific member of the TNFR superfamily. CD27 is expressed by virtually all mature T cells and by a subpopulation of B cells, mainly memory B cells. In mouse, CD27 has been found on nearly all thymocytes excluding a population of CD46-CD8-precursors. CD27 binds to CD70 and, through this interaction, plays an important role in T cell-B cell interaction. It has been reported that triggering CD27 plays an important role in the maturation of CD4+ and CD8+ effector cells. LG.8F10 cross-reacts with human and rat CD27.When the monoclonal antibody LG.3A10 is crosslinked on purified T cells, proliferation is increased. In the absence of crosslinking, the antibody inhibits CD27-CD70 interaction.
Product Categories/Family for anti-CD27 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
29,137 Da
NCBI Official Full Name
CD27 antigen
NCBI Official Synonym Full Names
CD27 molecule
NCBI Official Symbol
CD27
NCBI Official Synonym Symbols
T14; S152; Tp55; TNFRSF7; S152. LPFS2
NCBI Protein Information
CD27 antigen
UniProt Protein Name
CD27 antigen
Protein Family
UniProt Gene Name
CD27
UniProt Synonym Gene Names
TNFRSF7
NCBI Description
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. [provided by RefSeq, Jul 2008]
Uniprot Description
Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.
Research Articles on CD27
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Product Notes
The CD27 cd27 (Catalog #AAA5309240) is an Antibody produced from Hamster and is intended for research purposes only. The product is available for immediate purchase. The CD27 antibody reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD27 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP). FC/FACS: = 0.125 ug/test A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal /formance in the assay of interest. Researchers should empirically determine the suitability of the CD27 cd27 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD27, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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