Western Blot (WB)
(Western blot analysis of anti-AMPK alpha 1 C-term Pab in mouse liver lysate. AMPK alpha 1 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
Rabbit anti-Human, Mouse AMPK1 Polyclonal Antibody | anti-AMPK1 antibody
AMPK1, CT (5'-AMP-activated Protein Kinase, Catalytic Subunit alpha-1 Chain, AMPK Subunit alpha-1, PRKAA1) (FITC)
Gene Names
PRKAA1; AMPK; AMPKa1
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Purified by Protein G Affinity Chromatography.
Synonyms
AMPK1; Polyclonal Antibody; CT (5'-AMP-activated Protein Kinase; Catalytic Subunit alpha-1 Chain; AMPK Subunit alpha-1; PRKAA1) (FITC); anti-AMPK1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human AMPK alpha 1. Species Crossreactivity: mouse.
Purity/Purification
Purified by Protein G Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Applicable Applications for anti-AMPK1 antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:100-1:500
FLISA: 1:1,000
Applications are based on unconjugated antibody.
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the C-terminal region of human AMPK alpha 1 (KLH).
Conjugate
FITC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of anti-AMPK alpha 1 C-term Pab in mouse liver lysate. AMPK alpha 1 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
Western Blot (WB)
(Western blot analysis of anti-AMPK alpha 1 C-term Pab in mouse liver lysate. AMPK alpha 1 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
Western Blot (WB)
(AMPKalpha1 Antibody (C494) western blot analysis in SK-BR-3 cell line lysates (35ug/lane).This demonstrates the AMPKalpha1 antibody detected the AMPKalpha1 protein (arrow).)
Western Blot (WB)
(AMPKalpha1 Antibody (C494) western blot analysis in SK-BR-3 cell line lysates (35ug/lane).This demonstrates the AMPKalpha1 antibody detected the AMPKalpha1 protein (arrow).)
Product Categories/Family for anti-AMPK1 antibody
References
1. Zhang, Q.H., et al., Genome Res. 10(10):1546-1560 (2000). 2. Stapleton, D., et al., J. Biol. Chem. 271(2):611-614 (1996).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
64,009 Da
NCBI Official Full Name
PRKAA1 protein
NCBI Official Synonym Full Names
protein kinase, AMP-activated, alpha 1 catalytic subunit
NCBI Official Symbol
PRKAA1
NCBI Official Synonym Symbols
AMPK; AMPKa1
NCBI Protein Information
5'-AMP-activated protein kinase catalytic subunit alpha-1; ACACA kinase; AMPK alpha 1; HMGCR kinase; AMPK subunit alpha-1; tau-protein kinase PRKAA1; acetyl-CoA carboxylase kinase; AMP -activate kinase alpha 1 subunit; hydroxymethylglutaryl-CoA reductase
UniProt Protein Name
5'-AMP-activated protein kinase catalytic subunit alpha-1
UniProt Gene Name
PRKAA1
UniProt Synonym Gene Names
AMPK1; AMPK subunit alpha-1; ACACA kinase; HMGCR kinase
UniProt Entry Name
AAPK1_HUMAN
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Product Notes
The AMPK1 prkaa1 (Catalog #AAA6462703) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AMPK1, CT (5'-AMP-activated Protein Kinase, Catalytic Subunit alpha-1 Chain, AMPK Subunit alpha-1, PRKAA1) (FITC) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's AMPK1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:100-1:500 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the AMPK1 prkaa1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AMPK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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