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Flow Cytometry (FC/FACS) (Flow cytometric analysis of anti-human CD27 on human whole blood lymphocytes. Human lymphocytes were stained with anti human CD27-R023 (solid line) or rabbit IgG isotype control (dashed line).)

Rabbit anti-Human CD27 Monoclonal Antibody | anti-CD27 antibody

Recombinant Anti-CD27 Antibody (APC), Rabbit Monoclonal

Gene Names
CD27; T14; S152; Tp55; TNFRSF7; S152. LPFS2
Reactivity
Human
Applications
Flow Cytometry, Functional Assay
Purity
Protein A
Synonyms
CD27; Monoclonal Antibody; Recombinant Anti-CD27 Antibody (APC); Rabbit Monoclonal; CD27/TNFRSF7 Antibody (APC); Rabbit MAb; CD27 molecule; Anti-S152 Antibody; Anti-S152. LPFS2 Antibody; Anti-S152.LPFS2 Antibody; Anti-T14 Antibody; Anti-TNFRSF7 Antibody; Anti-Tp55 Antibody; anti-CD27 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
023
Specificity
Human CD27/TNFRSF7
Purity/Purification
Protein A
Form/Format
Liquid; Aqueous solution containing 0.5% BSA and 0.09% sodium azide
Concentration
2ul/Test, 0.1mg/ml (varies by lot)
Applicable Applications for anti-CD27 antibody
Flow Cytometry (FC/FACS/FCM)
Antibody Type
Recombinant Antibody
Immunogen
Recombinant Human CD27/TNFRSF7 protein
Conjugation
APC
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant human CD27 (rh CD27) and conjugated with APC under optimum conditions, the unreacted APC was removed.
Preparation and Storage
This antibody can be stored at 2-8 degree C for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of anti-human CD27 on human whole blood lymphocytes. Human lymphocytes were stained with anti human CD27-R023 (solid line) or rabbit IgG isotype control (dashed line).)

Flow Cytometry (FC/FACS) (Flow cytometric analysis of anti-human CD27 on human whole blood lymphocytes. Human lymphocytes were stained with anti human CD27-R023 (solid line) or rabbit IgG isotype control (dashed line).)
Related Product Information for anti-CD27 antibody
CD27, also known as TNFRSF7, is a member of the TNF-receptor superfamily limited to cells of the lymphoid lineage, and exists as both a dimeric glycoprotein on the cell surface and as a soluble protein in serum. As a type I transmembrane glycoprotein of about 55 kDa existing as disulfide-linked homodimer, CD27 has been shown to play roles in lymphoid proliferation, differentiation, and apoptosis.It has an important role in the generation of T cell immunity and is an robust marker for normal memory B cells. It is a T and B cell co-stimulatory molecule, the activity of CD27 is governed by its TNF-like ligand CD70 on lymphocytes and dendritic cells. The CD27-CD70 interaction is required for Th1 generation responses to differentiation signals and long-term maintenance of T cell immunity, and meanwhile, plays a key role in regulating B-cell differentiation, activation and immunoglobulin synthesis.
Product Categories/Family for anti-CD27 antibody
References
Drner T, et al. (2004) Correlation of circulating CD27 high plasma cells and disease activity in systemic lupus erythematosus. Lupus. 13(5): 283-9.Sahota SS, et al. (2009) CD27 in defining memory B-cell origins in Waldenstrm's macroglobulinemia. Clin Lymphoma Myeloma. 9(1): 33-5.Jiang J, et al. (2010) Reduced CD27 expression on antigen-specific CD4+ T cells correlates with persistent active tuberculosis. J Clin Immunol. 30(4): 566-73.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
939
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
29,137 Da
NCBI Official Full Name
CD27 antigen
NCBI Official Synonym Full Names
CD27 molecule
NCBI Official Symbol
CD27
NCBI Official Synonym Symbols
T14; S152; Tp55; TNFRSF7; S152. LPFS2
NCBI Protein Information
CD27 antigen; CD27L receptor; T cell activation antigen S152; T-cell activation antigen CD27; tumor necrosis factor receptor superfamily, member 7
UniProt Protein Name
CD27 antigen
Protein Family
UniProt Gene Name
CD27
UniProt Synonym Gene Names
TNFRSF7
UniProt Entry Name
CD27_HUMAN

NCBI Description

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. [provided by RefSeq, Jul 2008]

Uniprot Description

CD27: Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.

Protein type: Membrane protein, integral; Apoptosis; Receptor, cytokine

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: integral to plasma membrane; extracellular region; external side of plasma membrane

Molecular Function: protein binding; transmembrane receptor activity; caspase inhibitor activity

Biological Process: release of cytoplasmic sequestered NF-kappaB; response to ethanol; cell surface receptor linked signal transduction; positive regulation of JNK cascade; positive regulation of B cell differentiation; negative regulation of caspase activity; immunoglobulin mediated immune response; negative regulation of apoptosis

Disease: Lymphoproliferative Syndrome 2

Research Articles on CD27

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Product Notes

The CD27 cd27 (Catalog #AAA8100309) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-CD27 Antibody (APC), Rabbit Monoclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD27 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS/FCM). Researchers should empirically determine the suitability of the CD27 cd27 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD27, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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