Testing Data
(Mouse anti Chicken CD268 / BAFF-RPeripheral blood lymphocytes were separated by density centrifugation and first stained with the mouse anti chBAFF-R (clone 2C4) and then with goat anti mouse IgG1 secondary antibody conjugated to FITC, finally blocked with 10% normal mouse serum and followed by staining with the mouse anti chicken Bu-1a/b (clone AV20) conjugated to APC.Image courtesy of Sonja H¤rtle, Institute for Animal Physiology, University of Munich)
Mouse anti-Chicken CD268/BAFF-R Monoclonal Antibody | anti-CD268/BAFF-R antibody
MOUSE ANTI CHICKEN CD268 / BAFF-R
Reactivity
Chicken
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay
Synonyms
CD268/BAFF-R; Monoclonal Antibody; MOUSE ANTI CHICKEN CD268 / BAFF-R; anti-CD268/BAFF-R antibody
Host
Mouse
Reactivity
Chicken
Clonality
Monoclonal
Isotype
IgG1
Clone Number
2C4
Specificity
CD268 / BAFF-R
Form/Format
Purified IgG - liquid
Concentration
IgG concentration1.0 mg/ml (varies by lot)
Applicable Applications for anti-CD268/BAFF-R antibody
Immunohistochemistry (IHC) Formalin/Paraffin, Flow Cytometry (FC/FACS)
Application Notes
Immunohistochemistry: This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Reactivity Notes
Duck, Quail, Turkey
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Buffer Solution
Phosphate buffered saline
Preparation and Storage
Store at +4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch
Shelf Life: 18 months from date of despatch
Testing Data
(Mouse anti Chicken CD268 / BAFF-RPeripheral blood lymphocytes were separated by density centrifugation and first stained with the mouse anti chBAFF-R (clone 2C4) and then with goat anti mouse IgG1 secondary antibody conjugated to FITC, finally blocked with 10% normal mouse serum and followed by staining with the mouse anti chicken Bu-1a/b (clone AV20) conjugated to APC.Image courtesy of Sonja H¤rtle, Institute for Animal Physiology, University of Munich)
Testing Data
(Mouse anti Chicken CD268 / BAFF-RPeripheral blood lymphocytes were separated by density centrifugation and first stained with the mouse anti chBAFF-R (clone 2C4) and then with goat anti mouse IgG1 secondary antibody conjugated to FITC, finally blocked with 10% normal mouse serum and followed by staining with the mouse anti chicken Bu-1a/b (clone AV20) conjugated to APC.Image courtesy of Sonja H¤rtle, Institute for Animal Physiology, University of Munich)
Testing Data
(Mouse anti Chicken CD268 / BAFF-R, clone 2C4.Paraffin sections of bursal tissue were stained with the mouse anti chicken CD268 / BAFF-R antibody, clone 2C4 (2.5 μg/ml) followed by horse anti mouse Ig secondary antibody conjugated to biotin. Visualization was undertaken using a Vector ABC Kit and peroxidase activity was developed using 3.3´ diamino-benzidine-tetrahydro-chloride (DAB). Image courtesy of Sonja H¤rtle, Institute for Animal Physiology, University of Munich)
Testing Data
(Mouse anti Chicken CD268 / BAFF-R, clone 2C4.Paraffin sections of bursal tissue were stained with the mouse anti chicken CD268 / BAFF-R antibody, clone 2C4 (2.5 μg/ml) followed by horse anti mouse Ig secondary antibody conjugated to biotin. Visualization was undertaken using a Vector ABC Kit and peroxidase activity was developed using 3.3´ diamino-benzidine-tetrahydro-chloride (DAB). Image courtesy of Sonja H¤rtle, Institute for Animal Physiology, University of Munich)
Related Product Information for anti-CD268/BAFF-R antibody
Mouse anti Chicken CD268 / BAFF-R, clone 2C4, recognises the chicken homologue of human BAFF-R, known also as CD268 and TNFRSF 13C, a cell surface antigen expressed by avian B-cells. Chicken BAFF-R (B cell activating factor receptor) differs from mammalian BAFF-R in that it is produced in B-cells (Reddy et al. 2008) and is not expressed by chicken T-cells. In addition to chicken, clone 2C4 has been shown to cross react with several other commercially important avian species and as such is a specific marker of B-cells for research in domestic galliforms. Clone 2C4 is unique in that it can be used in formalin fixed paraffin embbeded tissues and is therefore of particular use on sections from infection studies.
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Product Notes
The CD268/BAFF-R (Catalog #AAA224838) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MOUSE ANTI CHICKEN CD268 / BAFF-R reacts with Chicken and may cross-react with other species as described in the data sheet. AAA Biotech's CD268/BAFF-R can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Formalin/Paraffin, Flow Cytometry (FC/FACS). Immunohistochemistry: This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections. Researchers should empirically determine the suitability of the CD268/BAFF-R for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD268/BAFF-R, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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