Mouse anti-Human CD261 Monoclonal Antibody | anti-CD261 antibody

Anti-Hu CD261 PE

Cat. Num. AAA1800552

• Gene Names: TNFRSF10A; DR4; APO2; CD261; TRAILR1; TRAILR-1
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Synonyms: CD261; Monoclonal Antibody; Anti-Hu CD261 PE; TNF receptor superfamily member 10a; DR4; APO2; TNFRSF10A TRAILR1; TRAIL receptor 1; TRAIL-R1; anti-CD261 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: DR-4-02
• Specificity: The mouse monoclonal antibody DR-4-02 recognizes an extracellular epitope of TRAIL-R1 (DR4), a human death receptor 4 expressed in most human tissues (spleen, peripheral blood leucocytes, thymus) and in a variety of tumour-derived cell lines.
• Purity/Purification: The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Form/Format: Stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide
• Concentration: 0.1 mg/ml (varies by lot)
• Sequence Length: 468

• Applicable Applications for anti-CD261 antibody: Flow Cytometry (FC/FACS)
• Application Notes: FC/FACS: QC tested
• Immunogen: Fusion protein containing the extracellular part of TRAIL-R1 and the constant part of the heavy chain of the human IgG1.
• Conjugation: PE
• Preparation and Storage: Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light.

Flow Cytometry (FC/FACS)

(Flow cytometry analysis (surface staining) of CD261-transfected HEK-293 cells with anti-CD261/TRAIL-R1 (DR-4-02) PE.)

Related Product Information for anti-CD261 antibody

TRAIL-R1 (CD261, DR4) is a type I transmembrane protein, also called TRAIL receptor 1. The ligand for this DR4 death receptor has been identified and termed TRAIL, which is a member of the TNF family. DR4, as many other receptors (Fas, TNFR1, etc.), mediates apoptosis and NF kappaB activation in many cells and tissues. Apoptosis, a programmed cell death, is a operating process in normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by coupled of certain cytokines (TNF family - TNF, Fas ligand) and their death domain containing receptors (TNFR1, Fas receptor).

NCBI and Uniprot Product Information

• NCBI GI #: 259906438
• NCBI GeneID: 8797
• NCBI Accession #: NP_003835.3
• NCBI GenBank Nucleotide #: NP_003835.3
• UniProt Accession #: O00220
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 10A
• NCBI Official Synonym Full Names: TNF receptor superfamily member 10a
• NCBI Official Symbol: TNFRSF10A
• NCBI Official Synonym Symbols: DR4; APO2; CD261; TRAILR1; TRAILR-1
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 10A
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 10A
• UniProt Gene Name: TNFRSF10A
• UniProt Synonym Gene Names: APO2; DR4; TRAILR1; TRAIL receptor 1; TRAIL-R1
• UniProt Entry Name: TR10A_HUMAN

NCBI Description

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL), and thus transduces cell death signal and induces cell apoptosis. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein. [provided by RefSeq, Jul 2008]

Uniprot Description

TRAIL-R1: Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF- kappa-B.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 8p21

Cellular Component: integral to membrane; plasma membrane

Molecular Function: protein binding; protease binding; death receptor activity; TRAIL binding; receptor activity; transcription factor binding

Biological Process: caspase activation; induction of apoptosis via death domain receptors; apoptosis; activation of NF-kappaB-inducing kinase; signal transduction