Rabbit CD261 Polyclonal Antibody | anti-CD261 antibody

RABBIT ANTI HUMAN CD261 (N-TERMINAL)

Cat. Num. AAA221577

• Gene Names: TNFRSF10A; DR4; APO2; CD261; TRAILR1; TRAILR-1
• Applications: Western Blot
• Synonyms: CD261; Polyclonal Antibody; RABBIT ANTI HUMAN CD261 (N-TERMINAL); anti-CD261 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item recognises the 57kD apoptosis related protein designated DR-4 (death receptor 4), also known as CD261.DR4 is a cell surface protein with a death domain that has functional similarities to TNFR1 and CD95. The ligand for DR-4 is TRAIL, and thus DR-4 has also been called TRAIL-RI. DR4 is expressed in most human tissues. In contrast to CD95, TNFR1 and DR3 it does not use FADD to transmit death signals.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0 mg/ml (varies by lot)
• Sequence Length: 468

• Applicable Applications for anti-CD261 antibody: Western Blot (WB)
• Application Notes: Western Blotting: Minimum Dilution: 1/500; Maximum Dilution: 1/1000
• Perservative Stabilisers: 0.02% Sodium Azide
• Immunogen: Peptide from N-terminus of human CD261 (DR4); Buffer Solution
• Antiserum Preparation: Antisera to CD261 (DR4) were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by ion exchange chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Western blot analysis for whole cell lysates from HeLa (H), K562 (K0 and Jurkat (J) cell lines probed with Rabbit anti Human CD261 (MBS221577))

Related Product Information for anti-CD261 antibody

MBS221577 recognises the 57kD apoptosis related protein designated DR-4 (death receptor 4), also known as CD261.DR4 is a cell surface protein with a death domain that has functional similarities to TNFR1 and CD95. The ligand for DR-4 is TRAIL, and thus DR-4 has also been called TRAIL-RI. DR4 is expressed in most human tissues. In contrast to CD95, TNFR1 and DR3 it does not use FADD to transmit death signals.

NCBI and Uniprot Product Information

• NCBI GI #: 259906438
• NCBI GeneID: 8797
• NCBI Accession #: NP_003835.3
• NCBI GenBank Nucleotide #: NM_003844.3
• UniProt Accession #: O00220; Q53Y72; Q96E62; A8K5I4
• Molecular Weight: 50,089 Da
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 10A
• NCBI Official Synonym Full Names: tumor necrosis factor receptor superfamily, member 10a
• NCBI Official Symbol: TNFRSF10A
• NCBI Official Synonym Symbols: DR4; APO2; CD261; TRAILR1; TRAILR-1
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 10A; TNF-related apoptosis-inducing ligand receptor 1; TRAIL receptor 1; TRAIL-R1; cytotoxic TRAIL receptor; death receptor 4; tumor necrosis factor receptor superfamily member 10a variant 2
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 10A
• UniProt Gene Name: TNFRSF10A
• UniProt Synonym Gene Names: APO2; DR4; TRAILR1; TRAIL receptor 1; TRAIL-R1
• UniProt Entry Name: TR10A_HUMAN

NCBI Description

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL), and thus transduces cell death signal and induces cell apoptosis. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein. [provided by RefSeq, Jul 2008]

Uniprot Description

TRAIL-R1: Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF- kappa-B.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 8p21

Cellular Component: integral to membrane; plasma membrane

Molecular Function: protein binding; protease binding; death receptor activity; TRAIL binding; receptor activity; transcription factor binding

Biological Process: caspase activation; induction of apoptosis via death domain receptors; apoptosis; activation of NF-kappaB-inducing kinase; signal transduction