EIF4EBP1 recombinant protein

Recombinant EIF4EBP1 protein

Cat. Num. AAA389435

• Gene Names: EIF4EBP1; BP-1; 4EBP1; 4E-BP1; PHAS-I
• Synonyms: EIF4EBP1; Recombinant EIF4EBP1 protein; Eukaryotic translation initiation factor 4E-binding protein 1;also known as 4E-BP1 or eIF4Ebinding protein 1; EIF4EBP1 recombinant protein

• Host: E Coli
• Form/Format: Recombinant EIF4EBP1 protein is supplied in 25mM Tris pH7.4, 300mM NaCl, 10% glycerol, 0.5mM TCEP.

• Protein Species: Human
• Tag: His-Tag
• Protein Details: Recombinant EIF4EBP1 protein was expressed in E Coli as the full length protein (accession number NP_004086.1) with an N-terminal 6×His-tag.
• Notes: This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data this product is shown.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant EIF4EBP1 protein 12.5% SDS-PAGE Coomassie staining M.W.: 14.51 kDa Purity: ? 90%)

Related Product Information for EIF4EBP1 recombinant protein

Background: EIF4EBP1 (Eukaryotic translation initiation factor 4E-binding protein 1,also known as 4E-BP1 or eIF4Ebinding protein 1) is a repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.

Product Categories/Family for EIF4EBP1 recombinant protein

Recombinant Proteins; Transcription

NCBI and Uniprot Product Information

• NCBI GI #: 4758258
• NCBI GeneID: 1978
• NCBI Accession #: NP_004086.1
• NCBI GenBank Nucleotide #: NM_004095.3
• UniProt Accession #: Q13541; Q6IBN3; B2R502; D3DSW8
• Molecular Weight: 12,580 Da
• NCBI Official Full Name: eukaryotic translation initiation factor 4E-binding protein 1
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 4E binding protein 1
• NCBI Official Symbol: EIF4EBP1
• NCBI Official Synonym Symbols: BP-1; 4EBP1; 4E-BP1; PHAS-I
• NCBI Protein Information: eukaryotic translation initiation factor 4E-binding protein 1; eIF4E-binding protein 1; phosphorylated heat- and acid-stable protein regulated by insulin 1
• UniProt Protein Name: Eukaryotic translation initiation factor 4E-binding protein 1
• Protein Family: Eukaryotic translation initiation factor 4E-binding protein
• UniProt Gene Name: EIF4EBP1
• UniProt Synonym Gene Names: 4E-BP1; eIF4E-binding protein 1; PHAS-I
• UniProt Entry Name: 4EBP1_HUMAN

NCBI Description

This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq, Jul 2008]

Uniprot Description

4E-BP1: binds to eIF4E, preventing its assembly into the EIF4F complex and inhibiting cap-dependent translation. Phosphorylation of 4E-BP1 disrupts this binding, activating cap-dependent translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the PI3 kinase pathway.

Protein type: Translation; Translation initiation

Chromosomal Location of Human Ortholog: 8p12

Cellular Component: nucleoplasm; protein complex; cytoplasm; cytosol

Molecular Function: protein binding; translation repressor activity; eukaryotic initiation factor 4E binding

Biological Process: negative regulation of translational initiation; response to ethanol; cellular protein metabolic process; TOR signaling pathway; translation; insulin receptor signaling pathway; translational initiation; gene expression; positive regulation of mitotic cell cycle; negative regulation of protein complex assembly; G1/S transition of mitotic cell cycle; lung development