Sequence Information
Pigment Epithelium Derived Factor (PEDF) Recombinant Protein | PEDF recombinant protein
Recombinant Pigment Epithelium Derived Factor (PEDF)
Gene Names
Serpinf1; Pedf; Sdf3; EPC-1; Pedfl; AI195227
Applications
SDS-Page, Western Blot, ELISA, Immunoprecipitation
Purity
> 95%
Synonyms
Pigment Epithelium Derived Factor (PEDF); Recombinant Pigment Epithelium Derived Factor (PEDF); PEDF recombinant protein
Host
E Coli
Purity/Purification
> 95%
Form/Format
Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Sequence
The target protein is fused with two N-terminal Tags, His-tag and S-tag, its sequence is listed below.
MHHHHHHSSGLVPRGSGMKETAAAKFERQHMDSPDLGTDDDDKAMADIGSEF-SQ NVPSSSEGSP VPDSTGEPVE EEDPFFKVPV NKLAAAVSNF GYDLYRLRSS ASPTGNVLLS PLSVATALSA LSLGAEHRTE SVIHRALYYD LITNPDIHST YKELLASVTA PEKNLKSASR IVFERKLRVK SSFVAPLEKS YGTRPRILTG NPRVDLQEIN NWVQAQMKGK IARSTREMPS ALSILLLGVA YFKGQWVTKF DSRKTTLQDF HLDEDRTVRVPMMSDPKAIL RYGLDSDLNC KIAQLPLTGS MSIIFFLPLT VTQNLTMIEE SLTSEFIHDI DRELKTIQAV LTVPKLKLSF EGELTKSLQD MKLQSLFESP DFSKITGKPV KLTQVEHRAA FEWNEEGAGS SPSPGLQPVR LTFPLDYHL
MHHHHHHSSGLVPRGSGMKETAAAKFERQHMDSPDLGTDDDDKAMADIGSEF-SQ NVPSSSEGSP VPDSTGEPVE EEDPFFKVPV NKLAAAVSNF GYDLYRLRSS ASPTGNVLLS PLSVATALSA LSLGAEHRTE SVIHRALYYD LITNPDIHST YKELLASVTA PEKNLKSASR IVFERKLRVK SSFVAPLEKS YGTRPRILTG NPRVDLQEIN NWVQAQMKGK IARSTREMPS ALSILLLGVA YFKGQWVTKF DSRKTTLQDF HLDEDRTVRVPMMSDPKAIL RYGLDSDLNC KIAQLPLTGS MSIIFFLPLT VTQNLTMIEE SLTSEFIHDI DRELKTIQAV LTVPKLKLSF EGELTKSLQD MKLQSLFESP DFSKITGKPV KLTQVEHRAA FEWNEEGAGS SPSPGLQPVR LTFPLDYHL
Sequence Length
417
Applicable Applications for PEDF recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Organism
Mus musculus (Mouse)
Expression System
Prokaryotic expression
Residues
Ser19~Leu389 (Accession # P97298) with two N-terminal Tags, His-tag and S-tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Reconstitution
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
Sequence Information
Sequence Information
SDS-Page
SDS-Page
Testing Data
Testing Data
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
47.0kDa
NCBI Official Full Name
pigment epithelium-derived factor
NCBI Official Synonym Full Names
serine (or cysteine) peptidase inhibitor, clade F, member 1
NCBI Official Symbol
Serpinf1
NCBI Official Synonym Symbols
Pedf; Sdf3; EPC-1; Pedfl; AI195227
NCBI Protein Information
pigment epithelium-derived factor; SDF-3; caspin; serpin F1; serpin-F1; alpha-2 antiplasmin; stromal cell derived factor 3; stromal cell-derived factor 3; pigment epithelium derived factor; serine (or cysteine) proteinase inhibitor, clade F, member 1; serine (or cysteine) proteinase inhibitor, clade F), member 1; serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1
UniProt Protein Name
Pigment epithelium-derived factor
UniProt Gene Name
Serpinf1
UniProt Synonym Gene Names
Pedf; Sdf3; PEDF; SDF-3
UniProt Entry Name
PEDF_MOUSE
Uniprot Description
PEDF: a secreted neurotrophic protein that induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. The N-terminal (AA 44-121) exhibits neurite outgrowth-inducing activity. The C-terminal exposed loop (AA 382-418) is essential for serpin activity. Extracellular phosphorylation enhances antiangiogenic activity.
Protein type: Secreted, signal peptide; Inhibitor; Secreted
Cellular Component: axon hillock; extracellular matrix; extracellular space; cell soma; axon; perinuclear region of cytoplasm; extracellular region; basement membrane
Molecular Function: serine-type endopeptidase inhibitor activity
Biological Process: positive regulation of neurogenesis; negative regulation of angiogenesis; short-term memory; negative regulation of inflammatory response
Research Articles on PEDF
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Product Notes
The PEDF serpinf1 (Catalog #AAA2011789) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Pigment Epithelium Derived Factor (PEDF) can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the PEDF serpinf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: The target protein is fused with two N-terminal Tags, His-tag and S-tag, its sequence is listed below. MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF-SQ NVPSSSEGSP VPDSTGEPVE EEDPFFKVPV NKLAAAVSNF GYDLYRLRSS ASPTGNVLLS PLSVATALSA LSLGAEHRTE SVIHRALYYD LITNPDIHST YKELLASVTA PEKNLKSASR IVFERKLRVK SSFVAPLEKS YGTRPRILTG NPRVDLQEIN NWVQAQMKGK IARSTREMPS ALSILLLGVA YFKGQWVTKF DSRKTTLQDF HLDEDRTVRV PMMSDPKAIL RYGLDSDLNC KIAQLPLTGS MSIIFFLPLT VTQNLTMIEE SLTSEFIHDI DRELKTIQAV LTVPKLKLSF EGELTKSLQD MKLQSLFESP DFSKITGKPV KLTQVEHRAA FEWNEEGAGS SPSPGLQPVR LTFPLDYHL. It is sometimes possible for the material contained within the vial of "Pigment Epithelium Derived Factor (PEDF), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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