Human Latent Transforming Growth Factor Beta Binding Protein 2 (LTBP2) ELISA Kit | LTBP2 elisa kit

Human Latent Transforming Growth Factor Beta Binding Protein 2 (LTBP2) ELISA Kit

Cat. Num. AAA2020850

• Gene Names: LTBP2; WMS3; GLC3D; LTBP3; MSPKA; MSTP031; C14orf141
• Reactivity: Human
• Synonyms: Latent Transforming Growth Factor Beta Binding Protein 2 (LTBP2); Human Latent Transforming Growth Factor Beta Binding Protein 2 (LTBP2) ELISA Kit; C14orf141; LTBP3; LTBP2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Latent Transforming Growth Factor Beta Binding Protein 2 (LTBP2).; No significant cross-reactivity or interference between Latent Transforming Growth Factor Beta Binding Protein 2 (LTBP2) and analogues was observed.
• Sequence Length: 1821

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Urine, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.133ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Latent Transforming Growth Factor Beta Binding Protein 2 (LTBP2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Latent Transforming Growth Factor Beta Binding Protein 2 (LTBP2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LTBP2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Latent Transforming Growth Factor Beta Binding Protein 2 (LTBP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Latent Transforming Growth Factor Beta Binding Protein 2 (LTBP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Latent Transforming Growth Factor Beta Binding Protein 2 (LTBP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Latent Transforming Growth Factor Beta Binding Protein 2 (LTBP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for LTBP2 elisa kit

Signal transduction; Infection immunity; Bone metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 50927279
• NCBI GeneID: 4053
• UniProt Accession #: Q14767; Q99907; Q9NS51
• Molecular Weight: 195,052 Da
• NCBI Official Full Name: Latent transforming growth factor beta binding protein 2
• NCBI Official Synonym Full Names: latent transforming growth factor beta binding protein 2
• NCBI Official Symbol: LTBP2
• NCBI Official Synonym Symbols: WMS3; GLC3D; LTBP3; MSPKA; MSTP031; C14orf141
• NCBI Protein Information: latent-transforming growth factor beta-binding protein 2
• UniProt Protein Name: Latent-transforming growth factor beta-binding protein 2
• Protein Family: Latent-transforming growth factor beta-binding protein
• UniProt Gene Name: LTBP2
• UniProt Synonym Gene Names: C14orf141; LTBP3; LTBP-2
• UniProt Entry Name: LTBP2_HUMAN

NCBI Description

The protein encoded by this gene belongs to the family of latent transforming growth factor (TGF)-beta binding proteins (LTBP), which are extracellular matrix proteins with multi-domain structure. This protein is the largest member of the LTBP family possessing unique regions and with most similarity to the fibrillins. It has thus been suggested that it may have multiple functions: as a member of the TGF-beta latent complex, as a structural component of microfibrils, and a role in cell adhesion. [provided by RefSeq, Jul 2008]

Uniprot Description

LTBP2: May play an integral structural role in elastic-fiber architectural organization and/or assembly. Defects in LTBP2 are the cause of primary congenital glaucoma type 3D (GLC3D). An autosomal recessive form of primary congenital glaucoma (PCG). PCG is characterized by marked increase of intraocular pressure at birth or early childhood, large ocular globes (buphthalmos) and corneal edema. It results from developmental defects of the trabecular meshwork and anterior chamber angle of the eye that prevent adequate drainage of aqueous humor. Defects in LTBP2 are the cause of microspherophakia (MCSPH). It is a rare disease characterized by smaller and more spherical lenses than normal bilaterally, an increased anteroposterior thickness of the lens and highly myopic eyes. Lens dislocation or subluxation may occur, leading to defective accommodation. Defects in LTBP2 are the cause of Weill-Marchesani syndrome 3 (WMS3). A rare connective tissue disorder characterized by short stature, brachydactyly, joint stiffness, and lens abnormalities. Belongs to the LTBP family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 14q24

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix

Molecular Function: heparin binding; protein binding; growth factor binding; calcium ion binding

Biological Process: transforming growth factor beta receptor signaling pathway; protein secretion; protein targeting

Disease: Microspherophakia And/or Megalocornea, With Ectopia Lentis And With Or Without Secondary Glaucoma; Glaucoma 3, Primary Congenital, D; Glaucoma 3, Primary Infantile, B; Weill-marchesani Syndrome 3